Suggest assay protocol for Glutathione reductase (GR) from plant samples?

08 August 2015 0 9K Report

We want to assay the activity of glutathione reductase in wheat leaves in response to the foliar pathogens.

Badges
Science topic

Similar topics
Microbiology
Assays

More Sudhir Navathe's questions See All

How to find Latitude and Longitude co-ordinates of different geographical locations?

I have list of ~3000 geographical locations from various parts of India. I want to find out their Latitude and Longitude co-ordinates. I want to know if there is any tool / software that will...

04 May 2018 1,215 13 View

What is relevance sequencing of pcr product for both forward and reverse primer?

I am getting PCR product sequenced for one gene. Is it necessary to to do sequencing with both forward and reverse primer? What is the relevance and benefit of doing this?

31 December 2017 3,019 4 View

I want article on plasmid borne genes and their expression?

I want to read about plasmid borne genes and their expression - avr, hie, vie and pat genes of bacterial plasmid and their expression. Is their any good review article to read about it?

03 April 2017 532 2 View

Is there any optional software to SyMAP?

I want to to do Synteny Mapping of some plant genes. SyMAP supports only Linux or McOSX. I need tool or software that runs on Windows OS

31 December 2016 1,094 3 View

What could be the role of reactive oxygen species in Hemibiotrophy of fungal plant interaction?

Any one can discuss or suggest the role of reactive oxygen species and its production during such type of interaction.

06 July 2016 2,718 3 View

How to do two way ANOVA in using Sigmaplot and get the graphs?

I have data set of 10 biochemical parameters at different time points obtained after inoculation of pathogen to plant. i want to analyse it using Two way ANOVA and get graph plotted over time...

04 May 2016 8,127 2 View

I want inhibitors of lignin in plants which i should prefer?

i found PAL inhibitor, L-a-aminooxy-b-phenylpropionic acid (AOPP), (1-amino-2-phenylethyl)phosphonic acid,a-aminooxyacetate, and CAD inhibitors N(O aminophenyl)sulfinamoyl-tertiobutylacetate and...

01 January 1970 2,451 0 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

Aspen Plus Simulation?

Greetings When one is to perform Simulation or modelling of Pyrolysis of Biomass Waste materials using Aspen Plus software, and one has both the macromolecules breakdown (Cellulose, Lignin and...

06 August 2024 5,023 1 View

Measuring the Intelligence of a Species?

Larger brains, which typically contain more neurons, store and transfer more information (Tehovnik and Chen 2015), but the precise relationship between number of neurons and information has yet to...

05 August 2024 1,238 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

Why aren't most metal oxides insoluble in water?

Why BaO (s) + H2O(l) ---> Ba 2+(aq) + 2 OH - (aq) if Oxygen loves owning all the electrons, why would it bind to hydrogen which competes with Oxygen for electron ownership more so than...

03 August 2024 5,392 1 View

How to Transform my handy-wavy explanation of why group I/II metals oxides in aqueous solution are basic into a rigorous argument?

I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...

03 August 2024 9,918 0 View

¿Are oxygen vacancies in semiconductors capable to change the band gap?

Dear all! Oxygen vacancies do raise negatively the Fermi level by increasing the negative charge in the semiconductor crystal structure. In the case of n-type semiconductors, I wonder if this...

30 July 2024 8,339 2 View

Hello Researchers, Can anyone guide me on estimating the Leaf Area Index (LAI) of wheat using only weather and soil parameters? Is it possible?

I need to estimate the LAI of a wheat crop using only weather and soil parameters. Can anyone please help me?

28 July 2024 4,886 2 View