How to find Latitude and Longitude co-ordinates of different geographical locations?

I have list of ~3000 geographical locations from various parts of India. I want to find out their Latitude and Longitude co-ordinates. I want to know if there is any tool / software that will...

04 May 2018 1,023 13 View

What is relevance sequencing of pcr product for both forward and reverse primer?

I am getting PCR product sequenced for one gene. Is it necessary to to do sequencing with both forward and reverse primer? What is the relevance and benefit of doing this?

31 December 2017 2,882 4 View

I want article on plasmid borne genes and their expression?

I want to read about plasmid borne genes and their expression - avr, hie, vie and pat genes of bacterial plasmid and their expression. Is their any good review article to read about it?

03 April 2017 264 2 View

Is there any optional software to SyMAP?

31 December 2016 945 3 View

What could be the role of reactive oxygen species in Hemibiotrophy of fungal plant interaction?

Any one can discuss or suggest the role of reactive oxygen species and its production during such type of interaction.

06 July 2016 2,615 3 View

How to do two way ANOVA in using Sigmaplot and get the graphs?

I have data set of 10 biochemical parameters at different time points obtained after inoculation of pathogen to plant. i want to analyse it using Two way ANOVA and get graph plotted over time...

04 May 2016 7,968 2 View

Suggest assay protocol for Glutathione reductase (GR) from plant samples?

07 August 2015 8,468 0 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

Does different dilution factors affect negatively on NH3 reading ?

I have a sample of treated water sample & asked to obtain ammonia conc. in sample using a machine with 5-25 ppm detection range. However, upon testing with different dilution factors, all...

27 February 2021 8,483 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

How to increase solubility of Poly acrylic acid in Dioxane solvent?

I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...

25 February 2021 2,225 5 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View