How to find Latitude and Longitude co-ordinates of different geographical locations?

I have list of ~3000 geographical locations from various parts of India. I want to find out their Latitude and Longitude co-ordinates. I want to know if there is any tool / software that will...

04 May 2018 1,023 13 View

What is relevance sequencing of pcr product for both forward and reverse primer?

I am getting PCR product sequenced for one gene. Is it necessary to to do sequencing with both forward and reverse primer? What is the relevance and benefit of doing this?

31 December 2017 2,882 4 View

Is there any optional software to SyMAP?

31 December 2016 945 3 View

What could be the role of reactive oxygen species in Hemibiotrophy of fungal plant interaction?

Any one can discuss or suggest the role of reactive oxygen species and its production during such type of interaction.

06 July 2016 2,615 3 View

How to do two way ANOVA in using Sigmaplot and get the graphs?

I have data set of 10 biochemical parameters at different time points obtained after inoculation of pathogen to plant. i want to analyse it using Two way ANOVA and get graph plotted over time...

04 May 2016 7,968 2 View

Suggest assay protocol for Glutathione reductase (GR) from plant samples?

07 August 2015 8,468 0 View

I want inhibitors of lignin in plants which i should prefer?

01 January 1970 2,311 0 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View