I want article on plasmid borne genes and their expression?

More Sudhir Navathe's questions See All

How to find Latitude and Longitude co-ordinates of different geographical locations?

I have list of ~3000 geographical locations from various parts of India. I want to find out their Latitude and Longitude co-ordinates. I want to know if there is any tool / software that will...

04 May 2018 1,215 13 View

What is relevance sequencing of pcr product for both forward and reverse primer?

I am getting PCR product sequenced for one gene. Is it necessary to to do sequencing with both forward and reverse primer? What is the relevance and benefit of doing this?

31 December 2017 3,019 4 View

Is there any optional software to SyMAP?

I want to to do Synteny Mapping of some plant genes. SyMAP supports only Linux or McOSX. I need tool or software that runs on Windows OS

31 December 2016 1,094 3 View

What could be the role of reactive oxygen species in Hemibiotrophy of fungal plant interaction?

Any one can discuss or suggest the role of reactive oxygen species and its production during such type of interaction.

06 July 2016 2,718 3 View

How to do two way ANOVA in using Sigmaplot and get the graphs?

I have data set of 10 biochemical parameters at different time points obtained after inoculation of pathogen to plant. i want to analyse it using Two way ANOVA and get graph plotted over time...

04 May 2016 8,127 2 View

Suggest assay protocol for Glutathione reductase (GR) from plant samples?

We want to assay the activity of glutathione reductase in wheat leaves in response to the foliar pathogens.

07 August 2015 8,584 0 View

I want inhibitors of lignin in plants which i should prefer?

i found PAL inhibitor, L-a-aminooxy-b-phenylpropionic acid (AOPP), (1-amino-2-phenylethyl)phosphonic acid,a-aminooxyacetate, and CAD inhibitors N(O aminophenyl)sulfinamoyl-tertiobutylacetate and...

01 January 1970 2,451 0 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

Where should the ARS sequence be introduced on a plasmid?

Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...

05 August 2024 1,573 4 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

How can I inoculate a lyophilized anaerobic bacteria into the culture medium without an anaerobic chamber?

There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...

05 August 2024 4,945 3 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

How effective is the Citi Bloc standard basket in enhancing the accuracy and comparability of international construction cost assessments?

Citi BLOC Standard Basket Definitions: A standardized unit representing a fixed basket of construction materials, labor, and equipment costs priced in various cities. Purpose: To create a common...

04 August 2024 8,997 1 View