I want to read about plasmid borne genes and their expression - avr, hie, vie and pat genes of bacterial plasmid and their expression. Is their any good review article to read about it?
Please see the following RG links.
Article Plasmid-borne prokaryotic gene expression: Sources of variab...
Article Expression characteristics of the plasmid-borne mcr-1 colist...
I have list of ~3000 geographical locations from various parts of India. I want to find out their Latitude and Longitude co-ordinates. I want to know if there is any tool / software that will...
04 May 2018 1,215 13 View
I am getting PCR product sequenced for one gene. Is it necessary to to do sequencing with both forward and reverse primer? What is the relevance and benefit of doing this?
31 December 2017 3,019 4 View
I want to to do Synteny Mapping of some plant genes. SyMAP supports only Linux or McOSX. I need tool or software that runs on Windows OS
31 December 2016 1,094 3 View
Any one can discuss or suggest the role of reactive oxygen species and its production during such type of interaction.
06 July 2016 2,718 3 View
I have data set of 10 biochemical parameters at different time points obtained after inoculation of pathogen to plant. i want to analyse it using Two way ANOVA and get graph plotted over time...
04 May 2016 8,127 2 View
We want to assay the activity of glutathione reductase in wheat leaves in response to the foliar pathogens.
07 August 2015 8,584 0 View
i found PAL inhibitor, L-a-aminooxy-b-phenylpropionic acid (AOPP), (1-amino-2-phenylethyl)phosphonic acid,a-aminooxyacetate, and CAD inhibitors N(O aminophenyl)sulfinamoyl-tertiobutylacetate and...
01 January 1970 2,451 0 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?
06 August 2024 9,618 3 View
Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...
05 August 2024 1,573 4 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
There are anaerobic gas pak jars and environment providers in the laboratory that provide anaerobic environmental conditions for the growth of anaerobic bacteria. However, there is no anaerobic...
05 August 2024 4,945 3 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
Citi BLOC Standard Basket Definitions: A standardized unit representing a fixed basket of construction materials, labor, and equipment costs priced in various cities. Purpose: To create a common...
04 August 2024 8,997 1 View