Lactose ?

Hello,

I should have phrased my question better. Sorry for that!

First of all, I am working on a exo-galactosidase which is active on ß-D- but not alpha-D-galactose-pNP. Therefore, I am calling my enzyme a ß-D-galactosidase.

I am happy with the pNP substrates (I do not need the fluorescent substrate) and I could test X-Gal and lactose BUT I would like to have different kinds of substrates in order to determine substrate specificity better (similar to what people do with exo-fucosidases using blood and milk oligosaccharides varying e.g. in linkage and DP).

I was looking in papers and the website for different chemical suppliers for potential substrates (other then lactose, XGal and pNP) but I could not find any. That is why I was posting the question here :)

I also do not know which substrates my enzymes might degrade since it comes from a marine bacterium. That is why I have not tested lactose yet. I am not looking for chromogenic substrates in particular but anything with terminal galactose and I already checked Carbosynth and Elicityl.

Anyhow, thanks Stjepan for your input!

Hey Stjepan,

this was exactly what I was looking for. Thank you sooo much :)