Substitute for NaCN to prevent oxidation of NADH?

21 November 2019 0 8K Report

I want to perform an enzymatic assay involving oxidation of NADH to NAD. However, I am not able to procure sodium cyanide (NaCN) to prevent background oxidation due to import restrictions. Is there any other alternative to NaCN to prevent oxidation of NADH?

Also, if I am comparing the efficiency of various enzymes, do I need to prevent background oxidation of NADH? Oxidation of NADH will be the same in all the samples, and hence, I can compare the efficiency of the various enzymes. Please let me know if my assumption is correct or not.

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