Brijesh Kumar

5 Questions 10 Answers 0 Followers

Questions related from Brijesh Kumar

Does L- and D-lactate inter-conversion happen in higher organisms?

While going through the KEGG pathway, I found lactate racemase enzymes which help in the interconversion of D- and L-lactate. I also found details about it on the Wikipedia (...

10 June 2019 3,254 0 View

What does extraction by ethyl acetate means?

I am synthesizing a compound. The protocol says, that the product should be washed with water, extracted using ethyl acetate and then purified by silica gel column chromatography. The product is...

21 March 2016 476 5 View

Can MMT assay be used to study metabolism in plant protoplasts?

I want to study the effect of certain compounds on the metabolic activity of plant cells. If I use MTT assay for plant protoplasts, will it work?

02 December 2015 806 2 View

How are TFs synthesized in response to stress?

In response to stress certain genes are induced. These genes are induced due to some transcription factors (TFs). So how are these particular TFs transcribed in response to that particular stress?...

19 November 2015 7,779 4 View

Positive controls for qPCR for Ca2+ and MAPK signaling in plants?

Can anyone tell me downstream genes for calcium and MAPK signaling cascade in plants that can be used as positive controls for qPCR?

21 October 2015 8,961 1 View