Brijesh Kumar

7 Questions 11 Answers 0 Followers

Questions related from Brijesh Kumar

Substitute for NaCN to prevent oxidation of NADH?

I want to perform an enzymatic assay involving oxidation of NADH to NAD. However, I am not able to procure sodium cyanide (NaCN) to prevent background oxidation due to import restrictions. Is...

21 November 2019 7,708 0 View

Does L- and D-lactate inter-conversion happen in higher organisms?

While going through the KEGG pathway, I found lactate racemase enzymes which help in the interconversion of D- and L-lactate. I also found details about it on the Wikipedia (...

10 June 2019 3,302 0 View

What does extraction by ethyl acetate means?

I am synthesizing a compound. The protocol says, that the product should be washed with water, extracted using ethyl acetate and then purified by silica gel column chromatography. The product is...

21 March 2016 512 5 View

Can MMT assay be used to study metabolism in plant protoplasts?

I want to study the effect of certain compounds on the metabolic activity of plant cells. If I use MTT assay for plant protoplasts, will it work?

02 December 2015 857 2 View

Is it possible to use 1-photon confocal instead of 2-photon confocal microscopy?

A protocol requires me to use two-photon confocal microscopy. What if I take the image with 1-photon confocal microscope? Will I not get the image at all? Quality of the image will vary, but I...

01 December 2015 6,166 3 View

How are TFs synthesized in response to stress?

In response to stress certain genes are induced. These genes are induced due to some transcription factors (TFs). So how are these particular TFs transcribed in response to that particular stress?...

19 November 2015 7,824 4 View

Positive controls for qPCR for Ca2+ and MAPK signaling in plants?

Can anyone tell me downstream genes for calcium and MAPK signaling cascade in plants that can be used as positive controls for qPCR?

21 October 2015 9,001 1 View