How long should be stable a functionalized CNT? and how can I increase its stability?

I have functionalized MWCNT via both acidic and noncovalent rout, For acidic treatment I have used H2SO4:HNO3 (3:1) while ultrasonication at 55 C, and in non-covalent I used Triton x100, but the...

27 January 2021 4,497 3 View

What is the Melting point criterion in a MD simulation?

I need a quantitative criterion, something other than structural and visual qualitative features of melting in the molecular dynamics simulations.

26 January 2021 9,096 5 View

Athena IE in surrogate modeling?

I was wondering if anyone has deployed Athena IE in surrogate modeling or metamodels for LCA. There are many research involving data-based models for predicting energy consumption, but I haven't...

23 January 2021 2,267 1 View

Graphene model in CST?

Hi everyone, I wanna know about creating the Graphene model in CST. In: Macros -> Materials -> Create Graphene Material for Optical Applications. What is the exact formula that is used in this...

25 December 2020 1,938 4 View

How can I extract MCPD in oil and determination with GC-MS?

Hello everybody I am going to extract MCPD in oil ,but I havent pressurized liquid extraction. How can I extract MCPD in oil and determination with GC-MS?

20 December 2020 7,389 2 View

How can the SAR image be divided into two looks using the spectral diversity method?

Hello I'm working on SAR Images Co-registration.I want to divide my image into two looks with the spectral diversity method. But I did not find its complete formulas in any article. Does anyone...

13 December 2020 3,270 4 View

Are any Ph.D. programs or research labs working on Extraterrestrial habitat and greenhouse?

I am looking for opportunities to expand my research. Please let me know if you have any information. Just a note, I am available for collaboration on papers and projects.

19 October 2020 5,887 1 View

I want to know to calculate green and blue water in SWAT model,if the data is entered on a daily basis, can I receive the output on a daily basis too?

Hi everyone need your help.thank you

22 September 2020 8,704 4 View

How can i increase the pH of synthesic sea water to 12?

How can i increase the pH of sea water to 12? Because when i adding NaOH to seawater the pH does not go above 10 and begins to precipitate.

12 September 2020 564 9 View

How to measure the approximate mass of the attached layer to QCM-D silica coated sensor using the stitch function?

I am trying to form a covalently-bound layer of MPTMS on a QCM-D silica coated substrate, and want to use the stitch function in QTools to approximate the mass of the layer. I do not know which...

24 August 2020 2,076 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

I am looking for an antibody to Arc protein for use in immunohistochemistry with rhesus monkey brain tissue?

Looking for a well-validated antibody to detect Arc protein expression in paraformaldehyde fixed brain tissue using immunohistochemistry. Ideally this antibody would have been published on using...

07 February 2021 8,457 1 View

How should I go about evaluating cytoplasmic and nuclear Arginase-1 activity?

Hello! I've stained some bright field Arginase-1 Hematoxylin slides, but I'm having a hard time finding any computed histology predicates for evaluating the intensity of the cytoplasm verses the...

03 February 2021 3,039 1 View

Antibody dilution for IHQ in OCT frozen tissue is more similar to IHC in Paraffin-embedded samples or to Immunocytochemistry?

31 January 2021 8,678 3 View

Any tips on Fab blocking of mouse tissue?

Hi, everyone, I am working on immunostaining of bone marrow and growth plates of mice. And I have to use an mouse monoclonal Ab to do this staining. However, with only secondary goat anti-mouse...

21 January 2021 9,826 3 View

Antigen retrieval for neural cell marker on frozen brain tissue?

Hi, I'm having problems with the Recombinant Anti-MAP2 antibody from Abcam (Recombinant Anti-MAP2 antibody [EPR19691] (ab183830)) antibody on my frozen, 4%PFA perfused, mice brain tissue. I...

10 January 2021 4,002 5 View

How to approach quantification of cytoplasmic immunofluorescent signal in adipocytes?

Any ideas on how to quantify a non-nuclear immunofluorescent signal in adipocytes? I had the idea to do a watershed on inverted image to "locate" the center of the empty space where lipid droplets...

05 January 2021 5,420 1 View

Can you recommend a protocol for immunohistochemistry of mouse brain sections using anti-MAP2?

Current protocol does not give clear images.

04 January 2021 1,614 3 View

I need to know about immunohistochemistry steps?

Immunohistochemistry staining. Tissue Fixation during IHC. Using DAB. blocking step. controls group.

23 December 2020 5,768 2 View

ISO antibody against BFP for FFPE IHC?

Suggestions and recommendations welcome. Any usage/protocol experience would also be helpful/appreciated. Cheers

22 December 2020 599 3 View