I'm looking for an immunohistochemistry protocol for Substance P (SP) in the Ventral Pallidum. I would appreciate any help.
Dear Mahsa, would you mind to tell your preference for the specs you are trying to localize Substance P? (species?) IHC - on paraffin embedded material or frozen sections? IHC, ImmFLUOR, etc. .... Thank you for specifying....
I have a Karl-Fischer reagent (for volumetric titration, which is a one-component reagent) with a titer of 5mg/ml. All references unanimously say the Karl-Fischer solution loses its capability...
02 July 2024 5,044 3 View
Greetings and Regards I have a question about bibliometric articles about public health or nursing. If any of the researchers have experience or knowledge in this matter, please tell me. Thank You
15 June 2024 2,795 1 View
Hi everyone Is there any guideline for method validation for cosmetic? I wanna know about criteria for that.
31 May 2024 1,990 2 View
I am working on a project to detect genetic mutations in lung cancer using machine learning algorithms and I am new to genetics and medical sciences. Can I use RNA as input to the algorithm for...
13 May 2024 5,726 6 View
Generally when they want to use hydrogel for MTT assay, firstly they fix hydrogel with glutaraldehyde then use for MTT assay. so, now, i have self healing hydrogel and its have crosslinker in its...
11 May 2024 6,310 1 View
Hi Have a nice day I want to determination PAAs and PAHs in tatoo. Would you present and introduce methods to this?
02 April 2024 6,772 0 View
"Hi, I gasify the polyethylene terephthalate granules and mold them using a hot press at a temperature of 260 degrees Celsius to prepare a bulk sample. However, after molding with the hot press,...
31 March 2024 8,668 0 View
I have a dataset from lung cancer images with 163 samples (2D images). I use the fine-tuning of deep learning algorithms to classify samples, but the validation loss did not decrease. I augmented...
21 March 2024 8,344 6 View
Hello, I want to dissolve polyphthalamide in a solvent. I tested different solvents, formic acid, toluene, DMSO, alcohol-based solvents, etc. under high temperature and time, but it did not...
12 March 2024 3,030 1 View
Hi. I need to remove all microplastics of a wastewater sample without changing any physicochemical properties of the sample. Can filtration by Whatman filters (for example Whatman 589/3 with...
08 March 2024 8,722 3 View
I am doing immunohistochemistry of the c-Fos protein and I have a budget problem. For context, the experiment has been done over 3 years prior to my arrival and I have 40 brains done and 100 left....
29 July 2024 5,961 2 View
Hello everyone, A collaborator sent us his protocol in order to be able to use his immunohistochemistry kits. But one step contains PBS + 0.3% H2O2 + 0.1% sodium azide. Knowing the dangerousness...
23 July 2024 6,314 3 View
We have mice brains that were over-fixed due to old PFA used during perfusion. Thus, the synapses are no longer being labeled by the Synaptophysin (SY38) mAB. which works perfectly every other...
17 July 2024 7,767 3 View
I'm looking to immunostain some brain tissue collected previously. The animal was *not* perfused prior to tissue collection. Will the presence of blood interfere with all staining methods...
14 July 2024 177 4 View
Hi all, I would like to know if it is possible to use Bovine Serum Albumin (BSA) in my blocking solution for immunofluorescence muscle fiber typing. The primary antibodies' host is mouse. The...
07 July 2024 7,967 3 View
What are the latest, modern and effective treatments for treating thalassemia and sickle cell anemia? If you find new research, please send it to me
28 June 2024 7,297 3 View
In the context of doctoral research, I utilized immunohistochemical (IHC) analysis to quantify the expression levels of the Ki-67 proliferation marker. While the application of this test and...
25 June 2024 7,825 2 View
Hello! I've recently been trying to optimise immunofluorescent staining of mouse meningeal tissue, and I'm having trouble mounting it just because it's so thick. We use prolong to mount and...
05 June 2024 624 2 View
I need to do immunohistochemistry in mouse brain, particularly in neurotensin-cre mouse line.
22 May 2024 3,565 7 View
Although I tried to perform western blotting with protein extract from RBCs, I could not detect the interest of protein due to red background (maybe, heme or hemoglobin ) all over the lane. I...
15 May 2024 6,182 1 View