What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

What can I do if DNA OD260/280 is either 1.6 or higher than 3?

26 January 2021 7,830 4 View

How do you multiple impute item scores and perform a linear regression on factor scores?

I am developing a questionnaire and first performing an exploratory factor analysis. After I have the final factor structure, I plan on regressing the factor scores on some demographic covariates....

18 January 2021 883 8 View

How do I design an extra long linker?

I'm trying to insert a linker between two proteins that is almost 100 amino acids in length, does anyone have any experience designing such a linker? I plan to order a gene block from IDT and...

17 December 2020 7,812 1 View

How to perform statistic analysis for age range between two cohorts?

Hi, I was reading the paper (MicroRNA Expression, Survival, and Response to Interferon in... ). In Table 1, I'm wondering how did the authors calculate a p-value of 0.29 for Age? The age in Table...

20 October 2020 2,276 5 View

Intron contetion and primer selection?

Dear all I am trying to clone a gene, which only contains only "one intron" in the middle part. I treat the extracted RNA with DNAse and use verso cDNA kit, so the DNA contamination ie...

10 September 2020 7,022 4 View

Does DTT affect cell lysis?

Hi, I am currently working with S. cerevisiae. general rule of thumbs for yeast cell lysis is to use 0.2M NaOH incubation followed by lysis using high urea buffer (containing 100mM DTT) at 70oC...

05 September 2020 6,915 3 View

Can we use pig imune serum to perform a passive immunization in mice?

We have vaccinated pig with antigens and then, we would like to know whether we can use mice to perform a passive protection assay by using the pig immune sera. Do you know pig macrophages can...

15 April 2020 2,510 2 View

Serum starvation before transfection and cell doubling time?

Hi, everyone I have a simple question about timespan of serum starvation and transfection. Recently I work on U-2OS cells which have doubling time around 20 hours, and before I transfect U-2OS...

15 March 2020 3,293 4 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

Where can I find public datasets with Covid-19 patient information?

Hi, I'm looking for public datasets that have CT, X-ray information, or medical records for patients with Covid-19. Does anyone have suggestions?

28 February 2021 4,760 3 View

How to get rid of somebody pretending to be the author of a publication?

One of the publication I co-authored has the author's name in abbreviation. Then, somebody whose nothing to do with that paper acquired it as her work. To whom in this RG shall I ask for a hand,...

27 February 2021 4,206 4 View

Can I put a figure that was drawn by Bio-Render in publication paper?

I want to publish paper in journal but I have figures which have drawn with bio-renders, can I put them in the paper or which other software I can use to draw them?

27 February 2021 8,179 1 View

Why is My firend's profile not visible?

Hello, I created a profile for my friend on google scholar. I have added a verified email address. I marked the profile as public. Formerly his profile was visible. However, now, When I type his...

24 February 2021 8,680 3 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

How was your experience with Biomedical and Biotechnology Journal?

How was your publication experience with BBRJ in terms of peer review and reviewer comments. Did you find it fair and transparent ?

23 February 2021 3,810 6 View

How to minimise the risk of being abused the profile of potential participants tagged in a social media post?

Dear colleagues, I got this one in one project I did ethics review. It's great to hear about your thoughts/experiences. "Doing an open recruitment online (i.e., via Facebook) requires public...

22 February 2021 3,322 3 View

Research Gate Does it help researches to publicize their scientific work ?

In my opinion, the RG site is useful in solving scientific problems and shows the researcher's ability. Besides, the separation of the H-index from the self-citations and the overall index is an...

22 February 2021 3,894 6 View

I can't edit my publication, the "edit" is blurred. How can I edit it?

I have entered a wrong question mark in my publication and added "Forthcoming", I want to edit the title of my publication, but the "EDIT" is blurred, I can't edit it. Any suggestion? thank you.

18 February 2021 1,279 3 View