How to make sure that ultrasonic lysis of E.coli is done completely?

What's the criteria of comolete lysis of E.coli when breaking them using ultrasonic? What phenomena can help to determine if the cell are broken completely? From smell, transparence of the lysis...

07 January 2021 1,911 3 View

What is the difference between "pesticides" and "insecticides"? Are they same?

What is the difference between "pesticides" and "insecticides" in English? Are they the equivalent replacement when used in text? I am an ecological researcher who particularly concerns the...

04 January 2021 6,230 37 View

Ungrounded CPW fed slot-ring patch antenna not behaving as an antenna in CST simulation?

Greetings everyone, I am attempting to model and simulate a slot-ring patch antenna fed by ungrounded CPW in CST, although the S11 is fine, the e-field and h-field monitors shows strange...

10 October 2020 2,228 2 View

Can we perform MOX/MSTFA derivatization in Eppendorf tubes?

Hi there! Recently I am planning to work on metabolomics using GC-MS, where derivatization is a crucial step. Here, I am wondering if that possible to derivatize extract samples with both MOX and...

24 September 2020 9,695 1 View

What is the right time course when using cycloheximide to inhibit protein synthesis?

I'm going to use cycloheximide to inhibit the synthesis of a membrane protein in HEK-293T cells, but I'm not sure the right time that needed to treat cells without influencing its viability. I...

21 September 2020 4,321 2 View

How to eliminate the warning of 'position not found when parsing line' during DAMASK simulation with ABAQUS/standard?

Hi, I am trying to use the ABAQUS/standard solver to simulate the single crystal deformation behaviour with DAMASK framework. The materials is BCC ferrite with hooke elasticity and phenopowerlaw...

10 December 2019 4,667 1 View

What is the yellow/brown salt crystal during protein crystallization?

During protein crystallization screening, we encountered several types of proteins grew yellow stick crystals with a reservoir containing 0.1 M BIS-TRIS pH 6.5, 2.0 M Ammonium sulfate. However,...

09 January 2019 2,188 1 View

Does anybody know any company manufacture cyclic olefin polymers: COP (except Zeonex/Zeon)?

Want to try COP from different vendors! Is there any one in China? Thanks!

24 April 2018 8,736 2 View

What is a quick and easy screen for visualizing the presence of gold nanoparticles?

Hi all, I am trying to design a hypothetical screen to find additional genes that are responsible for C. metallidurans' ability to reduce gold ions (Au(I) and Au(III)) to solid gold nanoparticles....

12 February 2018 7,511 2 View

One virus may have two different kinds of particle?

15 February 2017 676 10 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

Why there are two peaks while using the mie plot of Silver nanoparticle?

Hi, I am trying to learn the theoretical modelling of silver nanoparticle using the Mie scattering theory. After that, I discovered a software named Mieplot developed by Philip Laven, where it...

25 February 2021 7,493 6 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Color of spiro-ometad film?

What does the color of a spiro-ometad film indicate? I got purple, green, red, even black color of the spiro film. Is there anything we can get from the preliminary color analysis(e.g. oxidation...

21 February 2021 1,831 4 View