Structures of SYBR dyes

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Dataset for evaluating seismic performance of bridges?

I want to train neural networks to evaluate the seismic performance of bridges, but the papers online are all based on their own databases and have not been published. Where can I find the...

29 March 2024 6,946 1 View

How the result of random regression model in dairy cow to apply in dairy industry?

I am currently exploring the practical applications of random regression models in the dairy industry. Through these models, it is possible to derive coefficients from the lactation curve,...

08 January 2024 3,758 0 View

Do you think that Newton's gravity has a repulsive force?

Comparing gravitational field to electromagnetic field, the charge has the same sex and the opposite sex, but there are two kinds of "force"? Is there any gravity and any repulsion? What if...

20 December 2023 4,961 21 View

Does anybody know the structure of RiboGreen and OliGreen for quantification of RNA?

Laurie J. Jones; Stephen T. Yue; Ching-Ying Cheung; Victoria L. Singer (1998). RNA Quantitation by Fluorescence-Based Solution Assay: RiboGreen Reagent Characterization. , 265(2), 368–374....

31 October 2023 167 0 View

MGB vs QSY/BHQ in quenching efficiency, which one is better?

I know MGB can bind and stabilize the duplex, but it should be low in extinction coefficient and Max. Ab.(nm). Does anybody know the quenching efficiency of MGB comapred to QSY/BHQ? Foy...

19 September 2023 7,009 0 View

The binding affinity between extracellular vesicles (EVs) in serum and IgG in serum, how strong is it?

I would like to know if the binding between extracellular vesicles (EVs) in serum and immunoglobulins is strong or not.

02 August 2023 6,316 1 View

How to calculate diatom dissolution index (DDI)?

Ryves D.B. (2006, 2009) proposed the use of formulas for calculating the Diatom Dissolution Index (DDI). However, determining the dominant species of diatoms and classifying them into levels (1-4)...

01 August 2023 1,610 3 View

What is the right way for baseline correction in general?

During my time processing results curves, I frequently need to correct the baseline before peak fit, and intensity comparison. This thing happens to XRD, XPS, Raman, and many other data...

02 May 2023 9,586 3 View

For the anisotropic material, how to use the dielectric tensor calculates transmission rate?

I have the thickness data and dielectric tensor from ellipsometry

06 April 2023 7,770 2 View

About phi scan?

Dear all, can you please help me with a quesiton in XRD phi scan? Let's say we have a cubic Si (111) substrate, and I choose (100) plane to do the phi scan, how many peaks can we expect to...

28 March 2023 2,633 3 View

Could dyes amplify the spectrum of light to a specific wavelength?

I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...

05 August 2024 3,289 1 View

What is the problem with these tissue culture plants?

All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.

01 August 2024 588 4 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,891 4 View

How gold procurement?

Please note it

29 July 2024 4,920 3 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,005 0 View

How gold procurement?

https://www.cleanteqwater.com/resources/case-studies/gold-mine-australia/

29 July 2024 5,160 1 View

How to quantify the fluorescence intensity of only the merge area of an image using ImageJ software?

Dear All: I have to quantify the fluorescence intensity of cells that are fluorescent to two different markers, so i'm interested in finding out if this is possible. Previously I have...

22 July 2024 8,769 1 View

Waht is the effect of isoelectric point on MB dye photodegradation by ZnO nanoparticles?

Recently I have submitted an article regarding MB dye degradation by ZnO nanoparticle. I received a suggestion to include the discussion on the effect of pH. I want to know what is the impact of...

19 July 2024 4,867 1 View

What are the key risks associated with foodborne pathogens in imported leafy greens, the impact on public health and effect on regulatory framework?

I am currently writing a systematic review

15 July 2024 9,176 1 View

How do i go about my research which is about the integration of solar and wind power to produce green hydrogen?

Let me say I have a solar plant in operation and I want to build up a wind power plant, and later make a hybrid so that I could power the electrolyser as load for green hydrogen production.

14 July 2024 9,547 4 View