I want to store my cell supernatant for further use in assays for detection of steroid in it. Hence what is the optimal temparature in this case for storage of supernatants so that the steroids are not degraded?
Steroids are generally stable. You can even add to the samples organic solvent for extraction and leave it on room temperature for a while.
Kind regards,
Marko Vitas
If you want to store for long time, I suggest u to add 1% glycerol or DMSO.
Best Wishes;
M.M. Zeitoun
Thank you all for your advices.
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
how to get links for copyrights for papers?
06 August 2024 7,410 1 View
I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...
31 July 2024 2,033 1 View
Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...
30 July 2024 841 2 View
Am trying to develop a lesson/lab to determine and compare antioxidant properties of soybeans of various colors. Preference would be low tech and low cost. Any assistance is greatly appreciated.
25 July 2024 7,498 2 View
In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....
24 July 2024 8,076 3 View
Their is new research published in the biological control journal, and I am one of the authors. I want to add it to my account at researchgare. How can I do this?
21 July 2024 7,545 0 View
I have read that it is better to use other research methods alongside document analysis for triangulation, although it can also be used as a stand-alone method.
18 July 2024 4,218 0 View
I am conducting a study on Effectiveness of e-content on academic achievement of secondary school science student.
17 July 2024 5,633 4 View
I am simulating interlocking / dry stacked masonry in Abaqus. I am using a friction coefficient of 0.6 for my model as it is stated in the previous research as well as in the base paper. The...
16 July 2024 5,047 0 View
What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...
04 August 2024 6,265 1 View
I’m having difficulty achieving high RNA integrity in my samples. Although the 260/280 and 260/230 ratios are satisfactory after RNA extraction, the RNA samples show signs of degradation when...
22 July 2024 155 4 View
I want to extract glycosides from my mangrove leaf samples, but my laboratory lacks an incubator capable of reaching temperatures above 60°C. The paper I am following specifies a minimum...
13 July 2024 3,686 0 View
We're studying the stability and fusion behavior of multiple PVC compounds in a Brabender. After a few trials, we set the temperature at 195 °C and the rotor speed at 70 rpm. Samples differ in...
12 July 2024 2,768 1 View
Hello, I am looking for (estimated) degradation rates of cellulose in a classic anaerobic digestion setup (meso or thermophilic, WWTP sludge, 30+ days of operation) to have an idea of the % of...
01 July 2024 5,867 5 View
In an antibiotic degradation experiment using metals carbon composites to activate PMS, the results doesn't fit pseudo first or second order kinetics however the degradation efficiency is very...
26 June 2024 6,941 3 View
Why soil pollution is caused due to excessive use of chemical fertilizers and excessive use of chemical fertilizers and pesticides leading to soil degradation?
22 June 2024 4,508 3 View
Expecting a technical reason
19 June 2024 4,495 0 View
I am working with electroluminescence (EL) imaging of photovoltaic panels and need to ensure that the modules are not damaged during testing. I understand that the bias current is usually set...
16 June 2024 5,514 1 View
why uv is done and why photocatalytic measurement is done although both use uv radiation
09 June 2024 8,750 1 View