21 November 2022 1 6K Report

After a culture period of 7 days with M-CSF, is it needed to still add M-CSF to the cells when you use them in a stimulation assay (for example stimulation with LPS, IFN, TNF)?

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Can I please get you to remove my photograph from my profile?

I wish to remove my photograph from my profile the system does not allow me to do it I thank you

25 April 2023 2,023 3 View

Mouse bone marrow neutrophil stimulation with LPS? Isolation temperature and RNA extraction?

I am trying to isolate neutrophils from mouse bone marrow with percoll (62%). The goal is to stimualte the isolated neutrophils with LPS and afterwards lyse the cells for RNA analysis. I wonder...

15 March 2022 7,570 2 View

Using the Pfaffl method: Is it possible that the ratio of the calibrator does not equal 1?

Hi all, I have a question regarding calculations using the Pfaffl method. I have 3 groups per experiment: 1 control group (calibrator) and 2 treatment groups with 6 to 7 biological repeats per...

19 February 2021 8,757 2 View

How to combine 2 independent sample T tests with different group sizes in SPSS?

Hi, I carried out 2 experiments with the same experimental conditions Exp1 has 3 groups of 8 replicates Exp2 has 3 groups of 6 replicates Since I am interested in a dose-effect size, I did...

26 November 2020 7,736 3 View

Why do the axons of my hiPSC-derived neurons don't grow through the microgrooves of my compartmentalized chamber?

I am culturing human iPSC-derived neurons in compartmentilized chambers. I have run multiple experiments where axons grow nicely through the grooves. However neuronal cells from the same batch now...

03 March 2020 5,831 0 View

Does anyone know the composition of CultureOne supplement (ThermoFischer) or an alternative?

Since there is a shortage of the CultureOne supplement (ThermoFischer), I am looking for alternatives. I tried differentiation of NSC into neurons without the supplement but that doesn't look great.

08 April 2019 374 1 View

What staining is best to detect lignin in angiosperm stems embedded in LR-white?

I'm trying to determine the grade of lignification in the peduncles of different angiosperms using ImageJ. First I embedded the stems in paraffin and stained them with Etzold's solution (beautiful...

08 April 2019 8,983 2 View

Does anyone have experience with qPCR analysis on sorted Th17 cells?

For my project, I need to isolate Th17 cells out of skin and check gene expression via qPCR. Does anyone have some experience with this or knows of an alternative way to address this? All...

19 August 2016 7,791 3 View

Measurement invariance: How to identify your model?

When using CFA models to examine measurement invariance, one needs to identify these models by assigning a scale to the latent constructs. Two ways of doing this seems to appear frequently in the...

12 November 2013 3,567 4 View

Which BOOKS should all students in Spatial Planning have read?

For a MSc Course in Spatial Planning ('Reflections on planning and design practices') I am renewing the book list - with more contemporary, more diverse and more inclusive literature. What are...

01 January 1970 367 5 View

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Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

How long will macrophages remain in the M1 state after treatment with 100 ng/mL of LPS?

In my experiment, I treated RAW macrophages with 100 ng/mL of LPS. After 24 hours, I collected the supernatant and replaced it with fresh media. I then quantified the cytokine levels of TNF and...

01 August 2024 476 2 View

(treating astrocytes with LPS) Has anyone observed the recovery of astrocytes from LPS injury, a few hours after the treatment?

or can you recommend articles showing the differential effect of LPS in a time dependant manner?

28 July 2024 2,547 1 View

How do we pick data for determination of Validation Acceptance Criteria?

Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....

23 July 2024 7,292 3 View

Why am I observing very high Temperature and Pressure Increase using NPT Ensemble?

Hello everyone, I am running DFT with CP2K software at 1200 K, 1 bar with a periodic system of 64 Al atoms + 2 CO2 atoms. However, I am seeing my temperature slowly increase to >4000 K and my...

22 July 2024 3,177 0 View

Can anyone suggest the specification of the test tube used for the tube adherence method for biofilm assay?

when we grow biofilm in test tube, the pipette often do not reach near the content. so we have to decane it directly or use a smaller test tube.

17 July 2024 3,532 0 View

Trouble finding an anti-Digoxigenin antibody to perform Southern Blot technique?

I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...

15 July 2024 6,668 0 View

Techniques regarding protein-protein interaction in vitro?

What techniques or assays will be used when a purified lyophilized powder is purchased from a company to study protein-protein interaction?

15 July 2024 1,598 3 View

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