Stimulation BMDM cultures: need to add M-CSF?

21 November 2022 1 6K Report

After a culture period of 7 days with M-CSF, is it needed to still add M-CSF to the cells when you use them in a stimulation assay (for example stimulation with LPS, IFN, TNF)?

Malcolm Nobre

Hello Marlies Burgelman

You could refer to the research article attached below. (See Fig 2).

Article Isolation and culture of murine bone marrow-derived macropha...

Best.

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