Using the Pfaffl method: Is it possible that the ratio of the calibrator does not equal 1?

Hi all, I have a question regarding calculations using the Pfaffl method. I have 3 groups per experiment: 1 control group (calibrator) and 2 treatment groups with 6 to 7 biological repeats per...

19 February 2021 8,351 2 View

How to combine 2 independent sample T tests with different group sizes in SPSS?

Hi, I carried out 2 experiments with the same experimental conditions Exp1 has 3 groups of 8 replicates Exp2 has 3 groups of 6 replicates Since I am interested in a dose-effect size, I did...

26 November 2020 7,515 3 View

How to illustrate the occupations of different groups according to environmental gradients?

Hi! I have been working with cave invertebrates from semi-arid regions, and have observed some interesting responses from the community to variables at the local and regional level. I know which...

28 September 2020 1,592 7 View

What staining is best to detect lignin in angiosperm stems embedded in LR-white?

I'm trying to determine the grade of lignification in the peduncles of different angiosperms using ImageJ. First I embedded the stems in paraffin and stained them with Etzold's solution (beautiful...

08 April 2019 8,816 2 View

Is there any free software to draw signaling pathways?

30 March 2016 701 5 View

Measurement invariance: How to identify your model?

When using CFA models to examine measurement invariance, one needs to identify these models by assigning a scale to the latent constructs. Two ways of doing this seems to appear frequently in the...

12 November 2013 3,405 4 View

Can you suggest a sub-problem and possible objectives regarding to this thesis/research study?

03 November 2020 8,155 3 View

Image database of vegetation quadrats?

I am trying to develop a web teaching module (thanks to COVID) on plant community ecology. I am searching some database of photographs from plant quadrats to let students experience the whole...

14 October 2020 8,156 1 View

Is there a good way to do insect bioinventories without capturing and killing insects?

Dear entomologist colleagues, just wanted to find out if any of you have ever conducted and published on insect bioinventories without collecting insects? Is it defensible to do catch, ID and...

29 September 2020 7,660 16 View

Can someone let me have references with description of this aboriginal cross carved in rock?

I saw this cross in an NGS video and photographed the screen, but I did not learn where this cross was found. Seems to me that it was found in some island. In Cuba there is one very much similar...

25 March 2020 2,296 2 View

Recommendations of reading on photovoice (or auto-photography) and social-spatial difference?

I am interested in relationships between photovoice or auto-photography as research methods and social-spatial difference, either as captured in the photographs, or as embodied or lived by the...

23 January 2020 4,179 2 View

Any idea how this image was taken?

A student sent me this image and asked how it was done. My background is in optics, but it has been a number of years since I worked with surface interferometry. I am wondering if you can identify...

12 December 2019 8,423 4 View

Any idea how this image was taken?

A student sent me this image and asked how it was done. My background is in optics, but it has been a number of years since I worked with surface interferometry. I am wondering if you can identify...

11 December 2019 793 4 View

Is there any best way to differentiate between photograph taken at day light and night, other than comparing the pixel intensity values of images ?

I am trying to do it by plotting histogram of sample photographs.

13 September 2019 8,568 3 View

How can i decide the best annealing temperature of the primers (Gel electrophoresis photographs is attached here)?

I used four primers (Primer 2,4, 5 and 6) with 2 rice variety and tested its annealing temperature at 53, 54, 55, 56,57 and 58 degree centigrade on PCR. The photograph of the gel electrophoresis...

08 September 2019 7,061 5 View

Is there any best way to differentiate between photograph taken at day light and night, other than comparing the pixel intensity values of images ?

I am trying to do it by plotting histogram of sample photographs.

08 September 2019 404 3 View