Can I please get you to remove my photograph from my profile?

More Marlies E Alvarenga's questions See All

Stimulation BMDM cultures: need to add M-CSF?

After a culture period of 7 days with M-CSF, is it needed to still add M-CSF to the cells when you use them in a stimulation assay (for example stimulation with LPS, IFN, TNF)?

20 November 2022 6,107 1 View

Electrical schematic of Mass spectrometer Leak detection?

I need the schematic of Veeco MS-17 leak detector, it can be found on manual, if anyone have it, I will appreciate very much. Thanks. Regards,

30 October 2022 9,460 2 View

Mouse bone marrow neutrophil stimulation with LPS? Isolation temperature and RNA extraction?

I am trying to isolate neutrophils from mouse bone marrow with percoll (62%). The goal is to stimualte the isolated neutrophils with LPS and afterwards lyse the cells for RNA analysis. I wonder...

15 March 2022 7,570 2 View

Using the Pfaffl method: Is it possible that the ratio of the calibrator does not equal 1?

Hi all, I have a question regarding calculations using the Pfaffl method. I have 3 groups per experiment: 1 control group (calibrator) and 2 treatment groups with 6 to 7 biological repeats per...

19 February 2021 8,757 2 View

How to combine 2 independent sample T tests with different group sizes in SPSS?

Hi, I carried out 2 experiments with the same experimental conditions Exp1 has 3 groups of 8 replicates Exp2 has 3 groups of 6 replicates Since I am interested in a dose-effect size, I did...

26 November 2020 7,736 3 View

How to illustrate the occupations of different groups according to environmental gradients?

Hi! I have been working with cave invertebrates from semi-arid regions, and have observed some interesting responses from the community to variables at the local and regional level. I know which...

28 September 2020 1,773 7 View

Why do the axons of my hiPSC-derived neurons don't grow through the microgrooves of my compartmentalized chamber?

I am culturing human iPSC-derived neurons in compartmentilized chambers. I have run multiple experiments where axons grow nicely through the grooves. However neuronal cells from the same batch now...

03 March 2020 5,831 0 View

Does anyone know the composition of CultureOne supplement (ThermoFischer) or an alternative?

Since there is a shortage of the CultureOne supplement (ThermoFischer), I am looking for alternatives. I tried differentiation of NSC into neurons without the supplement but that doesn't look great.

08 April 2019 374 1 View

What staining is best to detect lignin in angiosperm stems embedded in LR-white?

I'm trying to determine the grade of lignification in the peduncles of different angiosperms using ImageJ. First I embedded the stems in paraffin and stained them with Etzold's solution (beautiful...

08 April 2019 8,983 2 View

Any suggestion of acid set for a complete estuarine sediment digestion in temperate area?

Dear colleagues, We need to digest a series of sediment samples extracted from mangrove rivers in Florianópolis-SC, Brazil, for FAAS analysis of heavy metal pollution.The objective is to evaluate...

26 January 2017 6,122 3 View

Which Peucedanum in Brno?

Queried plants formed nice clumps of sat green leaves resembling P. austriacum and P. alsaticum in April. Pale green stems and leaves appeared in the center soon, followed by pale green young...

01 June 2024 5,738 7 View

Can someone recommend a software (preferably free) that can help me enter and assed positional scan data to obtain inter-individual distances?

I have scan data of multiple individuals over a while and their positional photographs were taken at certain fixed instances during focal sampling. I want to obtain the inter-individual distances...

26 May 2024 2,597 2 View

How to count brown/brite adipocites on microscope?

How to count multilocular adipocytes on a slide with a white adipose tissue sample stained with H&E? I had done the count on the entire slide, but the articles put it in % of cells or...

10 April 2024 3,997 2 View

I want to know the scientific name of this species of "dragonfly" photographed by my friend the photographer Giacomo Pugliese in Oman last month?

25 March 2024 6,319 4 View

Seeking Advice on Cell Counting for SA Beta-Gal Staining?

I am currently conducting experiments involving SA beta-gal staining to detect senescent cells in my samples. I need guidance on the photography and cell counting aspects of my methodology....

26 February 2024 9,928 1 View

Q 1 - 2. Are you a fan of making predictions?

I photographed the "Herolds" сloud anomalies in Haifa. I indicate the time of the anomaly and the direction to the epicenter of the future earthquake. You can write when the earthquake will...

11 January 2024 3,784 9 View

This doesn't fit into science. Perhaps Buddhists are familiar with this?

This doesn't fit into science. Perhaps Buddhists are familiar with this? I ask you to let me know, maybe someone has encountered this. I personally photographed this at the end of the sunset in...

08 January 2024 5,060 3 View

How to photograph a stereoscopic sample of material using a confocal microscope ?

How to use a confocal microscope to photograph a material hydrogel microneedle sample of about 5mm*5mm*1μm (height) size, and how to place it? What are the precautions? Example has shown in the...

08 November 2023 4,243 0 View

Why it is seen that the low angle lines are single and the high angle lines are double in X-ray Powder Diffraction Photograph?

How can i understand the low angle lines and the high angle lines when noticed X-ray Powder Diffraction Photograph/Pattern? What are the significant of the lines? The picture is here!

25 September 2023 1,565 2 View

Where can I get Melanotus flavolivens?

Melanotus flavolivens has been widely reported to induce agarwood formation. I have not been able to find even a photograph of this fungi. I want to experiment with it. Where can I get...

15 August 2023 4,109 0 View

Stefan Van Heerden

You should be able to locate your profile page from the home page, can you find it?

There you ought to be able to edit your profile settings

Phạm Linh

I really don't know how to use this web. Sorry I can help Mrs.

Rosie Linham

click your profile picture in the top right, then 'your profile.' then you want to select the photo on your profile page. a pop-up should come up, and you should press 'delete photo' in grey underneath the black box.