Does anyone know the composition of CultureOne supplement (ThermoFischer) or an alternative?

09 April 2019 1 362 Report

Since there is a shortage of the CultureOne supplement (ThermoFischer), I am looking for alternatives. I tried differentiation of NSC into neurons without the supplement but that doesn't look great.

Francisco Mercado

CultureOne composition must be a secret. May be N-2 or B-27 supplements could be useful. N-2 promotes growing of neuronal cell lines, may be could be fine with NSC.

N-2 formulation (not at great detail) could be seen here:

https://www.thermofisher.com/mx/en/home/technical-resources/media-formulation.166.html

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