Marlies Burgelman

2 Questions 1 Answers 0 Followers

Questions related from Marlies Burgelman

Stimulation BMDM cultures: need to add M-CSF?

After a culture period of 7 days with M-CSF, is it needed to still add M-CSF to the cells when you use them in a stimulation assay (for example stimulation with LPS, IFN, TNF)?

21 November 2022 6,108 1 View

Mouse bone marrow neutrophil stimulation with LPS? Isolation temperature and RNA extraction?

I am trying to isolate neutrophils from mouse bone marrow with percoll (62%). The goal is to stimualte the isolated neutrophils with LPS and afterwards lyse the cells for RNA analysis. I wonder...

16 March 2022 7,571 2 View