Is there any simple experiment for measuring ROS in whole blood? Can I stimulate the whole blood with H2O2 for ROS generation ?
I have AFM scans of a uniformly textured surface of 16x16um size. I would like to obtain the slope distribution of the surface using Nanoscope, as I plan on trying to relate this parameter to the...
10 April 2024 5,024 2 View
Over the last 10 years, I have been using the Mendeley citation manager. Due to their new policy, it is not working as smoothly with MS Word. Therefore, I am looking for a good alternative.
10 January 2024 3,460 5 View
In CRISPR/Cas, we design guide to make double strand break in a specific site of the genomic DNA. I am curious to know, whether the gRNA-Cas9 complex has any possibility to bind to the messenger...
25 December 2023 630 1 View
If I want to knock out an essential gene from E.coli genome and replace it with a homologous gene from another genus. What should be the strategy I Should follow
25 October 2023 3,773 2 View
I am trying to simulate FSW in CEL using abaqus. I am trying to replicate exactly the same simulation of this paper :Coupled Eulerian Lagrangian finite element modeling of frict... . But the...
04 August 2023 3,782 0 View
in the model I am simulating, the mixture of ethylene glycol and water is flowing in the hollow fiber membrane (a cylindrical hollow tube made up of porous media). during the flow, the water in...
19 July 2023 7,116 1 View
I have been trying to simulate the Friction Stir Welding process in Ansys LS-Dyna using the SPH method. I am struggling to couple it thermally to find the temperature, add convection, etc. Please...
12 July 2023 8,181 0 View
I am trying to simulate Friction Stir welding using the CEL method. I have resolved all other errors except this particular error. Someone kindly help me with this. I have attached the CAE file.
11 July 2023 8,959 2 View
I am analyzing the gene sequence of my target gene in Brassica juncea. In that there is some ambiguities in the gene sequence which is appearing after few clear peaks in the sequence. I am...
27 June 2023 9,927 1 View
On the above mentioned modelling methods, which one is suitable for protein structure prediction for protein/gene families? Suggest some highly reliable modelling methods and software used for it.
12 June 2023 1,489 7 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View
In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
01 August 2024 4,403 4 View
I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...
31 July 2024 2,150 0 View
Hi all, My lab has Thermo Scientific™ Invitrogen™ EVOS™ FL Auto 2 Imaging System, and I was wondering if I will be able to use it with whole blood, whilst focusing on platelets? The idea would...
24 July 2024 9,337 3 View
Hello, good morning. I would like to know which plate I can use to infect fresh PBMC with virus and culture for 24 hours. I have tried using 12-well and 24-well plates, but many cells adhere and...
24 July 2024 2,158 2 View
hi every one I am making vector construction (for fusion proteins) and in this moment I wanna to amplification of ADAM17 prodomain with PCR. to yet, I couldn't amplified the ADAM17 prodomain with...
21 July 2024 8,660 1 View
Hi, I need help in detaching adherent monocytes from well plates. I isolated the PBMCs from buffy coat and I did CD14+ positive selection to collect only the monocytes. I let them adhere on my...
20 July 2024 7,922 2 View
These datasets will be used in the training of machine learning algorithms. Does anyone know any available data?"
17 July 2024 6,519 3 View
Type 2 diabetes can be managed and, in some cases, put into remission through proper diet and exercise, regardless of age. Here are some key points: Weight Management: Achieving and maintaining a...
07 July 2024 8,745 2 View
I'm simulating a magnetohydrodynamic problem of blood flow using lattice Boltzmann method in lattice units and I need to convert the magnetic conductivity to lattice units. The physical value is...
07 July 2024 5,243 0 View