How to ensure the sterility of the BD FACS aria for sorting samples thats going to be re infused to the patient after sorting
Can you do that?
Because all the antibodies you tag the cells with will undergo intracellular signaling...
What sort of scenarios would you do this for? Sounds really cool if possible!
Dear members, i am a researcher in the stem cell field, 4 years experience in operating a flowcytometer, i had worked with BD FACS calibur, BD FACS Aria and Beckman coulter FC 500 flowcytometers,...
05 June 2010 7,848 4 View
Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...
02 March 2021 1,999 5 View
Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
01 March 2021 3,867 3 View
Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...
25 February 2021 9,602 4 View
Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...
25 February 2021 3,443 4 View
New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...
21 February 2021 3,278 3 View
I would appreciate any comments on the following problem. I need to calculate mean fields (e.g. pressure, velocity and vorticity), for which I activate Trn Stats tab in CFX-Pre. However, what I...
21 February 2021 8,548 7 View
Hello everyone, hope all is good. I want to analyse multi-phase fluid flow through pipe, which software is best to do so ?
21 February 2021 4,230 8 View
I have tried Idelchik's Handbook of Hydraulic Resistance 4th Edition (2007 page 300) formulas and across some range the formulas are reported valid for, the law of conservation of energy is...
20 February 2021 2,504 1 View
18 February 2021 4,214 3 View
Hello everyone, When determining cells in different stages of the cell cycle, cells are stain for Brdu and Propidium Iodide. I understand how PI can be used to discriminate between G1 and S/G2...
18 February 2021 3,920 3 View