Currently we are working on Alzheimer's disease. From literature we unable to get conclusion regarding the usage of coordinates, as i have find out lot of difference in the coordinates from article to article.
Ex 1: 4.8 mm anterior to posterior (AP) Bregma, 2.2 mm mid to lateral (ML), and 3.0 mm dorsal to ventral (DV).
Ex 2: −3.6 mm anterior‑posterior to the bregma, 2.4 mm lateral to the sagittal suture, 2.8 mm dorsoventral.
Ex 3: −0.8 mm antero-posterior from bregma and ±1.5 mm medio-lateral from the midline.
etc.,.
I will be using Amyloid beta and Streptozotocin as an inducing agent.
What is the name of the structure of the brain in which you want to inject the Amyloid?
It depends on the region of interest. In our study we used 2 months old rat and performed stereotactic administration in the hippocampus.
First-in-Rat Study of Human Alzheimer's Disease Tau Propagation
The coordinates are as follows:
Anterodorsal hippocampal injection (A/P: −3.6 mm, M/L: ±2.0 mm, D/V: −2.3 mm)
@ Robin - we are targeting dorsal CA1 and
Thank you santosh
Most individuals have targeted the rodent dorsal hippocampus for learning and memory work, since of course the case of H.M., disregarding the sloppy monkey work that quickly followed trying to replicate H.M.'s amnesia. The work picked up again with David Olton's hippocampal disconnection lesion work in the mid-seventies, using the radial-arm maze, fining that the dorsal hippocampal lesion was just as effective as entorhinal and dorsal hippocampal lesions or entire hippocampal lesions. LTP also had its heyday in the dorsal hippocampus,regardless of whether the perforant-path Dentate or CA3 to CA1 pathways were examined. Possibly most relevant is Richard Morris' work of the 80s onward, shifting from hippocampal lesions to intrahippocampal infusion of N-Methyl-D-aspartate (NMDA) receptor antagonists, A-Phosphono-valeric Acid (APV), used to block both LTP and learning. Coordinates from this work in the Wmaze may be the most relevant. Encompasing his J.Neurosci. publications and his slew of Nature papers.
Matching strain and size is important, and Morris' has a J. Neurosci. paper where India Ink was also infused intrahippocampally to catch the spread of the infusion and document it in Histology to show restriction to Hippocampus, as well sufficient spread within the dorsal hippocampus. Others, have used Prussian Blue reactions during histology to passing current through insulated injection cannulae to again mark the infusion sites.The intracerebral infusion work was important with the staining, beaus it permitted separation of mechanical damage from cannulae implantations from that of the infusate.
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