How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

FDC and FRC cell populations in mouse spleen?

I was trying to isolate fibroblastic reticular cells (FRC) and follicular dendritic cells from mice spleen, but kept getting poor yield. I found from literature that the frequency of FDC is around...

17 February 2021 3,506 4 View

Can anyone elaborate the method to measure arterial pulse wave velocity in research studies involving Sphygmocor??

Sphygmocor is the tonometry based instrument which is used to record generally radial pulse waves and then calculating various variables like Augmentation Index, SEVR etc. It is also used to...

02 February 2021 7,546 3 View

How to remove ORO (Oil red O) background precipitates?

Hi ORO experts, I'm struggling with ORO background precipitates in arterial tissues. The protocol I use works very well on human atherosclerotic plaques which are full of lipids and I don't...

13 January 2021 7,110 1 View

Can you please answer and share this questionnaire survey about Covid-19 ?

Dear colleagues, Please answer it, will take less than 2 minutes, Please find attached the link below :...

10 December 2020 1,611 6 View

I need Coronary Angiogram Images for Coronary Artery Disease detection?

I need Coronary Angiogram Images(CAI) publically available dataset. Coronary Artery Disease (CAD) is a condition of the heart due to atherosclerosis. Atherosclerosis is the narrowing of arteries...

07 December 2020 6,470 3 View

Has anyone seen this perivascular black stuff in rat brains?

This photo is from a reportedly healthy Long-Evans rat. Several of these animals have similar findings. No other sign of illness. Has anyone out there seen this?

19 November 2020 8,108 4 View

How can we optimize a water distribution system if we do not have the zones' topology (pipes graph )?

Hello everyone, My issue is about a water distribution system that I am working on a zone of the system where does not exist any plan for the place of pipes. However, we have the place of...

14 September 2020 5,978 6 View

Can 70 degree (or 60 degree) hot air kill coronavirus on the cotton clothes? If yes, then what should be the duration of hot air blow?

I want to sterilise some items covered safely in dry cotton cloth packings. For what duration, should I blow hot air so that the cloth is free from lethal Coronavirus?

18 May 2020 2,803 2 View

What GIS tools can be used to analyze the global transmission and prediction of a new coronavirus pandemic, COVID-19?

What GIS tools can be used to analyze the global transmission and prediction of a novel coronavirus pandemic (COVID-19)? What methods and formulas can be used and what kind of geospatial tools can...

14 April 2020 9,225 46 View

CFD- how to model rigid body dynamics in a flow-driven valve?

Hello, I'm trying to simulate the flow in a pipe containing a bi-leaflet valve whose movement (the rotation angle) is driven by the flow at the inlet using Ansys CFX. I'm using an immersed solid...

23 March 2020 4,609 2 View