28 September 2023 0 2K Report

I would like to stain the artery with Hoechst dye. However, I am not able to visualize it with fluorescnet microscope. My question is it possible to stain the all artery without cryosectioning?

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Can breast cancer organoid dissociate with trypLE easly?

We have a problem during breast cancer organoid passaging. we can not disrupt compact structure of organoid. We tried trypsin (4-6min 37 C + pipeting ) but organoids disscociate partially. Then we...

10 June 2024 9,658 6 View

scf file for monoclinic system using Quantum espresso code?

I have calculated Y2SiO5 with monoclinic system (Y1-Y2 two site, unique Si site and O1, O2, O3, O4 and O5 five site). Thus, I have used these parameters as a input file descriptions; Monoclinic...

15 May 2024 9,072 2 View

Wyckoff positions+fractional coordinates ?

How can i adjust the fractional coordinates using Wyckoff positions. For example, 8c wyckoff positions with (0.25 0.25 0.25) fractional coordinates in cubic structure. best,

28 March 2024 7,171 0 View

VESTA error for charge density?

I have calculated charge density using this postprocessing for materials with cubic symmetry. But I have obtain VESTA image using .cube output file. Any comment for this...

24 March 2024 6,522 2 View

Pressure in quantum espresso?

Hello, I will apply hydrostatic pressure in quantum espresso for vc-relax and electronic band gap. I added the 'press' in cell section in the geometry optimization calculation. Its ok? and how...

27 February 2024 6,841 7 View

What is your mother's dog's name?

boncuk

09 December 2023 4,689 0 View

I cannot culture Pieris brassicae. Why don't my butterflies lay eggs?

I want to starting up a Pieris brassicae culture. I collected almost 10 egg packages from the cabbage fields different times. All larvaes are became butterflies but all butterflies were female....

10 November 2023 8,235 2 View

Are there any recent wearables for EDA/GSR measurements (possibly measuring skin temperature, heart rate, etc. as well)?

As Empatica E4 EDA/GSR wristband is discontinued, we are looking for a new device that can be used for scientific research. Ideally, a wristband and no electrodes since we would like to use it in...

09 October 2023 9,133 1 View

How can I design the miRNA primers for RT PCR?

Hello everyone, I would like to ask a question to manage my experiment. To decrease the budget of my experiment, I have been searching for designing miRNA primers for RT PCR. I could find just a...

14 May 2023 9,565 4 View

During the completion of the missing meteorological data; Can the daily sunshine duration data estimation be made with the IDW method?

I have meteorology datasets of 30 meteorology stations from 1992-2022. However, there are stations that are missing 20% of the sunshine time. I have completed these using IDV with the help of...

26 April 2023 306 1 View

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Senescence-associated beta galactosidase staining is False Positive in control group?

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