If the response is yes, how about the detection of ion channel proteins in homogenates by Western blotting?
I would like to ask if someone could help me to convert unit from OR per SD to 1mm for meta analysis. for example The age-adjusted OR per SD cIMT for MMSE
16 February 2021 6,476 2 View
Im new to DEGs and PCR data and i received a RT-qPCR data of interpolate corrected CT values of around 50 microRNAs . The dataset contains 5 different conditions each condition contains : - 3...
16 August 2019 3,403 3 View
A Journal required submission of an editable AMOS figure file but the AMOS output provide figure that is not modifiable. Any suggestion to handle this issue.
12 December 2017 2,237 1 View
Greetings, what is the estimated sample size for the hierarchical multiple regression analysis with 2 variables entered in the first step and one additional variable entered in the second step....
18 June 2016 874 9 View
If the homogeneity of regression slopes assumption for ANCOVA (no interaction between the covariate and the independent variable) was violated, what is the next step to perform the analysis. Is...
03 February 2016 7,818 5 View
We are conducting mixed methods using sequential exploratory design in two stages, the first stage consisted of three exploratory focus groups to inform the development of a cross-sectional...
13 March 2015 7,800 7 View
I'm trying to isolate viable single cell suspension from brains harvested from 2 month old mice/rat. I'm specifically looking for enzymatic methods of dissociation. I understand pappain works...
18 August 2014 4,633 4 View
Please advise.
26 January 2014 230 10 View
I recently stained for CB1R in brain slices from mice. The results showed clear expression in the shape of cells throughout the dorsal thalamus. I had stained for CB1R before, but had found very...
01 January 1970 1,494 3 View
Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...
03 March 2021 1,499 2 View
Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...
03 March 2021 6,299 3 View
I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...
02 March 2021 2,735 2 View
Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...
01 March 2021 2,215 2 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.
01 March 2021 4,306 1 View
Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.
01 March 2021 9,952 3 View
I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...
01 March 2021 7,675 3 View
I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...
01 March 2021 9,015 2 View
I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...
01 March 2021 8,079 2 View