I am planning to start antioxidant enzymatic assay of spinach plant. i will do this assay from the stem part of the plant. Can anyone suggest me the standard protocol for this assay
Waiting for your valuable suggestions
Optimized enzymatic colorimetric assay for determination of hydrogen peroxide (H2O2) scavenging activity of plant extracts. MethodsX. 2015; 2: 283–291
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4500160/
Review on in vivo and in vitro methods evaluation of antioxidant activity. Saudi Pharm J. 2013 Apr;21(2):143-52.
http://www.sciencedirect.com/science/article/pii/S1319016412000357
Hello
Antioxidant activity was determined by 1, 1- diphenyl-2- picrylhydrazyl (DPPH) free radical scavenging method. A 0.5 ml of plant extract was added to 2.5 ml of a 0.004 % ethanol solution of DPPH. Absorbance at 517 nm was determined after 30 min and the percentage inhibition activity was calculated from [(Ao-A1)/ Ao] ×100, where Ao is the absorbance of the control (DPPH solution) and A1 is the absorbance of the extract/standard.
Shachi Sing (2016)
Brand-Williams, Cuvelier & Berset (1995).
https://www.sciencedirect.com/science/article/pii/S0308814615302673
Best wishes
- Badges
- Science topic
- Similar topics
- Biological Science
- Botany
- Plant Biology
- Plant Biotechnology