As far as I saw, after staining lipid doplets with the Oil Red O solution. You must leave the wells in 100% isopropanol for at least 10-20 minutes and mesure the absorbance at 520 nm, right?
My question is related to the standard, I saw varieties between the standarization to the protein level and the absorbance of the crystal violet.
Which is better? Why that comparison with the crystal violet? There is any protocol to do this in relation with the protein concentration?
Thank you,
Sergio.
Could you please explain more on the procedure and samples you are trying to evaluate??
Sorry, the aim is to compare the adipogenic potentital of different stem cells. So to make an statistical analysis i would neet to do a semi-quantitative comparison through the extraction of lipid doplets in solution in order to measure absorbances.
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