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Questions related from Sergio Garnica Galvez
Hello every body, I want to perform a fibroblast-like colony unit formation or CFU assay or clonogenic assay for mesenchymal stem cells. Personally I am working with equine adipose-derived stem...
05 May 2017 508 5 View
Hello! I have 5 monoclonal antibodies with the isotype IgG1-kappa and 1 of them has the isotype IgG3-kappa. I want to discard inespecific binding by using isotypic negative controls. The question...
02 February 2017 2,689 6 View
As far as I saw, after staining lipid doplets with the Oil Red O solution. You must leave the wells in 100% isopropanol for at least 10-20 minutes and mesure the absorbance at 520 nm, right? My...
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Hello! I would like to ask about the quantification of sGAG after the chondrogenesis of mesenchymal stem cells. My principal doubt is how to extract the sGAG to performs an assay...
02 February 2017 6,813 6 View
