Is partial inhibition zone of bacteria considered as inhibition?

I work with the antimicrobial activity of natural products. I found that on Mueller Hinton agar plate one of the natural product is producing circular partial inhibition zone around it. Will that...

28 January 2020 2,364 11 View

Is partial inhibition zone of bacteria considered as inhibition?

I work with the antimicrobial activity of natural products. I found that on Mueller Hinton agar plate one of the natural product is producing circular partial inhibition zone around it. Will that...

31 December 2019 2,024 5 View

What is the scientific and accurate method to convert CGPA to Percentage in 4.0 scale systems?

Nowadays, so many people are concerned with converting CGPA to Percentage on a 4.0 scale. There is a common method in which they multiply the CGPA by 25 since one CGPA is equivalent to 25 in...

09 November 2019 9,816 8 View

I am working with a protein and trying to resolve it on Blue-Native PAGE. Can somebody suggest be a recipe for it?

I have been trying to perform Blue-Native PAGE using procedure from doi:10.1038/nprot.2006.62. However, the stacking gel does not solidifies even after two hours, at room temperature. Can...

04 April 2019 4,777 2 View

I am planning for Native PAGE for a protein with pI 8.5 - 8.7. What should be the minimum difference of pH of buffer to avoid electrode switching?

The Gel has pH 8.9. and the pH of loading dye is 6.67. What should be the pH of running buffer to allow smooth running of my protein into the gel. I prepared buffer of pH 8.49 but I was unable to...

25 March 2019 9,877 6 View

Any suggestion what's going on?

I submitted a manuscript on a novel and interesting finding on July 30, 2018 to a reputed journal. Following editorial review it went to peer-review. It is over 8 months, no decision yet. What...

24 March 2019 9,737 2 View

How to find the natural frequency of fixed thick glass?

Dear researchers, i am looking for equation to find the natural frequency for fixed thick glass. please suggest

28 November 2018 2,736 2 View

What are the advantages growth of GaN on graphene in optoelectronics?

I would like to write my research plan for my PhD on this topic.

21 April 2015 4,301 3 View

Is it compulsory/mandatory to examine the mediating effect in the conceptual framework that was analyzed utilizing AMOS?

Increased value of invertebrates - Mediating variable

08 April 2015 2,834 5 View

How is oil content estimated in mustard seed? Can anyone provide me a protocol regarding this?

I want to estimate the percent of oil present in mustard seed.

24 March 2015 2,233 7 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

No title

03 March 2021 5,360 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View