The Role of Town Planners in CAV Use?

Colleagues and I are about to begin a project investigating the role of town planners and local authorities in establishing the introduction of connected and autonomous vehicles (CAVs). What...

21 February 2021 2,247 3 View

Staining methods for skeletal muscle fiber typing?

does anyone know the best method to differentiate and quantify rapid fatiguing and fatigue resistant muscle fibers? the old method form the 1980's uses ATP staining which is quite labor intense?

21 February 2021 6,483 3 View

Does anybody have any knowledge of "dry" microbiological decontamination methods for frozen fruit?

I am looking for data on the implementation of a microbiological reduction step for frozen fruit pieces. I have information on cold plasma/UV but am unable to locate much data on gaseous methods...

24 November 2019 6,131 8 View

How can I purify a recombinant H 10 tagged N protein from a co-contaminant post IMAC SEC?

09 May 2016 6,765 4 View

Does an immortalized gastric epithelial cell line exist?

18 May 2015 10,013 1 View

Do you have a rotocol to make osteologic discs?

Does anyone have a protocol to make osteologic discs? E.g. hydroxyapitate discs. BD bioscience no longer produce them and the one protocol I have takes 3 weeks.

19 March 2015 5,358 3 View

What is the difference between the 'sociocognitive account' of discourse and cognition (van Dijk, 2006), and discursive Psychology's account?

The SC account of discourse argues against a direct contextual account in the analysis of discourse, proposing that such an account is limiting. Van Dijk argues that it is important - essential -...

17 November 2013 6,012 3 View

Can anyone please suggest a good protocol or assay to detect and quantify mature microRNAs (miRNAs) using real-time PCR instruments

17 February 2012 5,087 22 View

Functional skills assessment and AI

Does anyone know of any organisation that is using AI in functional skills (English and Maths) assessment (adults)? Extensive searches reveal no products or services on the market which leverage...

01 January 1970 1,986 6 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Do anyone here can help us in the field of molecular docking?

We have synthesized various metal complexes with different environment around the metals. Now to see the application of synthesized complexes we need this study also. we are also interested in...

01 March 2021 1,899 2 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View

How can we assess applied digital skills and soft skills through online examinations during COVID-19 semester?

The final qualification assessment for major programs is a strict regimen that involves different stages - oral and written exams, final project viva, internal and external review. How are ICT...

16 February 2021 1,762 8 View

What is the actual chemistry going on between iodate and ascorbic acid?

I am studying biochemistry at university, as part of the course we do several titration experiments. In our handouts, we were given the following half equations: IO3- + 6H+ + 4e- I+ +...

16 February 2021 8,731 1 View

An article on the Iranian state's civil liability in the COVID-19 matter needs supervision; Is anyone interested in collaborating?

Hello. An article on the Iranian state's civil liability in the COVID-19 issue has been written in collaboration with a friend and a comparative look at law and biology. The article is ready to be...

15 February 2021 2,906 2 View