I have sliced 50 mm cube in to 1mm slices. The cube has voids which are changing with depth. So if I stack the 2D slices I get a 3D structure. But i have a few questions :

a) how should i align the 2D stack and is there any method for good alignment.

b) how should i give the (Z-distance) distance between the images.

please share your opinion and please suggest me a good software, i.e open source.

regards

Harsha

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