Recently, I have worked on genetic diversity analysis in mango genotypes using SSR markers. I can do it well as it is diploid. However, I have a question in my mind. If I worked on the triploid or tetraploid organism for genetic diversity analysis using SSR markers, how I count allele bands in gel image and make input files for GeneAlex, Darwin and other statistical software.?
If anybody worked with the triploid and more allelic organisms, please help me.
hello Samaun Parvez
don't get confused with ploidy level and multiple allele. traits exhibition depends on allelomorph of candidate gene not on its ploidy level.
solution for your confusion is go for binary coding (presence or absence) in Darwin.
ex. for scoring
sample
Primer 1 (3 allele) Primer 2 (3 allele)
210 bp 220bp 250 bp 310bp 320bp 350bp
1 0 1 1 1 1
1 1 1 1 1 1
1 1 1 0 1 1
similarly in case of GenAlEx (based on band weightage)
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