Is GAPDH a suitable reference gene for qPCR in transfected cells?

I transfected Huh 7.5 cell lines with vector pDG461which harbors Cas9. I performed qPCR to make sure that Cas9 is expressed in my cell lines. I used GAPDH as a reference gene in qPCR and my...

11 July 2020 7,139 11 View

Would you please introduce me a functional and updated protocol's sources for biotechnology research ?

Hi everyone I am looking for a precise and comprehensive source for protocols of laboratory techniques which are used in biotechnology or cell culture and related subjects .Do you know guys any...

21 May 2020 889 8 View

Why is a DNA partial sequence (16s rRNA) placed in a separate branch in phylogenetic tree?

Dear all, I have some bacteria DNA partial sequences (16s rRNA). They sequenced only by 9 F primer. Their length is under 1100 bp. When I draw phylogenetic trees for some of them by MEGA, my...

23 April 2020 9,203 8 View

What is the effect of surrounding medium, e.g; Acidic nature, on spectral variation of LSPR for the Au nanoparticles?

I want to know origin of spectral shift in my plasmonic sensor when medium becomes more and more acidic.

08 October 2019 4,247 3 View

What is the best protocol for operating protein SDS -PAGE and Western blot ? Is there any video to learn more about details and trouble shooting?

hello every one I am looking for a really precise protocol for doing protein SDS PAGE and also Western blot and of course a good source for how to deal with their trouble shooting . any kind of...

23 August 2019 2,104 5 View

How is stabilizing NeoRez R981 Polymer with Triethylamine?

I was reading the patent " Method of providing a substrate with a hydrophilic coating and substrates, particularly medical devices, provided with such coatings", and in one of the examples, it...

21 December 2018 4,397 3 View

What is the best source for learning Comparative Genomics ?

Hi I am looking for a really functional source for learning more about details on the topic of Comparative Genomics .I would be appreciated to introduce me couple of them .

16 November 2018 2,647 9 View

How to calculate concentration of drugs in small intestine?

Does anyone know if there is any formula I can use for calculation of a drug concentration (knowing oral dose) in small intestine and/or other parts of GI? Thanks

20 February 2018 2,352 1 View

I have synthesized ZnO particles with a unique morphology. How can I stimate the thickness of the particles?

It seems that these particles are formed in the shape of flakes with nanosize thickness.

15 May 2017 6,716 1 View

What is aripiprazol

Please tell me what is aripiprazol?

04 January 2012 7,098 15 View

Physics: Why do we describe light by referring to its wavelength, but microwaves and radar pulses by referring to their frequency?

In physics, the description of the properties of light generally refers to its wavelength (nm), while in the range of microwaves and radio waves we refer to the frequency of a signal (e.g. kHz or...

03 March 2021 1,682 3 View

Is there any effect of different colours of light (white, red, yellow, blue etc.) on the rate of photosynthesis? If yes, why?

Light is inevitable for the process of photosynthesis reaction. Naturally sunlight plays the vital role for this process. Light may be of different colours like white, red, yellow, blue etc....

02 March 2021 7,890 2 View

Is stone a sustainable material? Is it a sustainable approach to use natural stone waste in architecture?

I'm designing a zero-waste restaurant and considering using marble waste to make some tables and a bar countertop. My clients think it's not "sustainable enough" and I would like to have more...

01 March 2021 4,326 7 View

In an eye with Tyndall, does each dot correspond to an individual cell?

When you observe the presence of Tyndall in an inflamed eye: Does each of the small floating dots correspond to an individual cell or to an aggregate of cells? Does a conventional slit lamp have...

27 February 2021 6,326 2 View

How can I obtain time series data by country on night-time light (brightness) ?

Hi everyone, Please, how can I obtain time series data by country on night-time light (brightness) ? I've tried, but so far all I can find are maps. I am looking for numerical data, especially in...

24 February 2021 8,625 4 View

What are difference between laser diodes, simple light emitting diodes and power light emitting diodes when operated in the visible spectrum ?

I want to illuminate a scene (small space) using LED to capture photographs or videos of some tiny objects inside the space. But am not sure if I should use a Laser LED, or simple LED or power...

22 February 2021 4,240 4 View

Why the nucleic acid gel melted after running for 30min?

Hi all, I proceed with nucleic acid gel electrophoresis recently several times, but a wried phenomenon is they always melted after running over 30min. My gel concentration is 1% percentage...

22 February 2021 9,896 3 View

How to interpret this FTIR spectrum of pyrolyzed hydrochar?

20 February 2021 2,840 3 View

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The regenerated cellulose film was prepared by zinc chloride/H2O system. The zinc chloride had been washed away in the regeneration process, and the wet film which was not dried had uniform...

18 February 2021 9,538 1 View

How do I plot Pressure against crank angle degree in Fluent?

18 February 2021 8,390 3 View