I am a graduate student in a protein biology lab and I am pregnant. I started doing trizol extraction from tissue and one of the steps is sonication.
Is sonication within a fume hood safe for pregnancy? Or should I switch to bead beating?
Good morning, I am trying to produce a recombinant protein. Unfortunately I one of the two restriction enzymes that I use do not cut at the end of the MCS. After the digestion 15 nucleotides of...
17 July 2024 2,787 4 View
I'm looking to immunostain some brain tissue collected previously. The animal was *not* perfused prior to tissue collection. Will the presence of blood interfere with all staining methods...
14 July 2024 177 4 View
Hi everyone, I am currently writing my masters thesis about Strategic Sponsor Acquisition in Youth Women's Soccer. The aim of the study is to identify potential sponsors for a specific youth...
04 July 2024 9,001 0 View
Hi i want to post this in various groups, how do i do that ? hey, please do my survey when you get the chance, it will take 15 minutes tops x I am looking for UK secondary school teachers to...
30 June 2024 9,594 2 View
We're using LB media, 1mM IPTG, and letting it incubate at 18°C over night. Will adding glucose or something else to the media increase bacterial expression?
06 June 2024 6,071 1 View
Hi I am trying to set up the reactions with my plasmid, insert and RE. Usually the standard final volume is 20 uL or 50 uL (it depends on the protocol provided by the manufacturer) with 1 ug of...
28 May 2024 1,105 3 View
Hi everyone, I'm having trouble with my hydroponic cultures for Arabidopsis thaliana, since lately I've started having algae contamination on them. I have tried using several sterilization...
21 May 2024 4,840 1 View
I'm a doctoral student with early experiences with recombinant protein synthesis. Since I still have to start my experiment and I do not yet know the quantities of DNA and plasmid that I will...
19 May 2024 5,171 3 View
What is recommended as a strong and current reference of qualitative phenomenological analysis ?
16 May 2024 2,142 1 View
I am looking for a some chapter or book related to the statistical analysis of mRNA transcriptomics. Thank you;)
09 May 2024 6,543 2 View
I am currently researching the impact of environmental toxins on children's health and would greatly appreciate insights from experts in the field. If you are an expert or researcher working on...
02 August 2024 8,361 0 View
I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...
30 July 2024 7,077 1 View
Hello, I want to ask regarding exosome protein quantification method. Is it necessary to lyse exosomes before measuring their protein contents using BCA assay kit? Or can I just straight away...
23 July 2024 4,603 4 View
I synthesised a nanocarrier by conjugation MOF (MIL-100) with folic acid for use in chemotherapy but the samples didn't dissolve completely in aqueous solution or DMSO. Instead, they only...
15 July 2024 2,414 1 View
Hello I have prepared metal nanoparticles and did zeta potential. Normally there is a single peak in sample but now there are 3 peaks. I prepared sample for analysis first I prepared 10 mg/mL...
30 June 2024 1,063 4 View
Hey all what are the ways by which an encapsulated drug inside BSA may escape from its encapsulation. We had synthesised curcumin encapsulated BSA by desolvation method followed by centrifugation...
23 June 2024 8,785 5 View
I have truck tire particles that I am using in my column experiment. I am trying to do size determination using the Malvern Zetasizer. My sample concentration is 0.1 mg/L, i.e., 10 g of sample was...
22 June 2024 8,857 6 View
Prevention Methods are Effective Against Teenagers Pregnancy
21 June 2024 6,321 10 View
What parameters are important in this process?
17 June 2024 4,463 0 View
Hello, I would really appreciate some help interpreting the output from my simple mediation analysis using PROCESS macro in SPSS please. For context, the X predictor is severity of nausea and...
13 June 2024 9,597 3 View