How to measure acid values of unsaturated polyesters?

I've been using the standart AATM 109-01 (2006) procedure to determine the acid values of unsaturated polyesters. In this procedure solutions of Phenolphthalein and Potassium Hydroxide in etanol...

11 November 2020 7,768 6 View

CAS solution whit PIPES ?

To prepare CAS solution for siderophores assay, the protocol use anhydrous piperazine. Can I make this solution using PIPES ? And how? Thanks The protocol i'm following is:...

08 November 2020 2,751 2 View

Does anyone have a reliable protocol to assess cell viability in commercially available GelMA (Gelatin methacryloyl) ?

Hello all, I am trying to asses the viability of stem cells in GelMA (gelatin methacryloyl). Most articles make their own GelMA. However, our team bought a commercially available one. The thing...

13 September 2020 9,086 1 View

Which are the best loading controls for Western Blot excluding the cytoskeleton ones?

I need to use a loading control for Western Blot that neither includes a cytoskeleton protein or a protein that interacts directly with the cytoskeleton. My issue is that I incubate cells with...

27 August 2020 2,735 11 View

Does anyone know or can resolve this kind of Internal Error on Anylogic?

I am currently working on a Urban Traffic Simulation on Anylogic and sometimes when the simulation is running, this error appears and everything stop: "Exception during discrete event...

23 May 2020 8,461 0 View

What is the header of the Gaussian input file for calculating two distinct molecules?

I'm doing simulations with two different molecules, but it always ends up falling into the same problem, it seems that the Gaussian doesn't balance the molecules when I calculate the cation and...

03 March 2020 7,750 2 View

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03 March 2020 4,189 1 View

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Hello, i need to generate a few parameters of excitation states in a series of molecules. I am using g09. I have a opensuse (linux) with 24 cores, Dell setup (i believe it configures a...

14 February 2020 9,488 5 View

Is anyone interested in setting up a distance research group on agricultural risk management?

I am looking for researchers who are interested in the theme "agricultural risk management" to do a research group at a distance. Each addresses the experiences of local risk management, from...

08 December 2019 8,879 29 View

Are there any articles about impulsive buying and grandparents?

Hello! I'm taking an internship in Social Psychology Laboratory and researching Impulsive Buying. However, it seems that little research gathers information about these two variables together. Me...

07 October 2019 4,982 5 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View