At that moment, I am counting my results about Micronucleus Assay. I have a lot of images of MDA-MB-231 dyed with Giemsa. I want to know some things:

· When you have a cell with a nucleoplasmic bridge, how would you clasificate it? As a mononucleated or binucleated?

· When you count micronucleus, you count only micronucleus in binucleated cells? Or you use it the micronucleus that appears in mononucleated cells?

· What is te main criteria that you use for value the damage in the micronucleus assay? I am dividing the number of micronuclei by the number of binucleated cells

· Do you think that is important to count another markers as nuclear buds or apoptotic cells?