I used Treg Suppression Inspector (Anti-CD2, Anti-CD3 and Anti-CD28) for Lymphocyte Stimulation, but after 3 Days I see low proliferation? What,s the problem?
Hi Christoph
I am working with Human Lymphocytes.
I reverse transcribed the RNA to the cDNA. Then I stored the cDNA in -20 freezer but after several months the volume of the cDNA decreased to 0.1. What is the reason?
05 July 2021 7,698 1 View
Dear Friends I use BD compbeads for compensation of dyes spill over in BD FACSCalibur flow cytometer. I can't set forward scatter and side scatter of single color compbeads to have a gated beads...
01 February 2019 3,260 3 View
I want to extract mitochondrial DNA from human cells. Can I use usual DNA extraction kit?
04 May 2014 6,692 1 View
Thanks in advance.
31 December 2013 2,648 6 View
19 September 2013 1,791 1 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Are there any statistical methods to justify your sampling technique using SPSS or AMOS?
05 August 2024 9,153 4 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View