I want to extract mitochondrial DNA from human cells. Can I use usual DNA extraction kit?
My colleague regularly uses the Qiagen DNeasy Blood & Tissue Kit to extract mitochondrial DNA for sequencing work, but there is also the QIAamp DNA micro kit for smaller samples.
I reverse transcribed the RNA to the cDNA. Then I stored the cDNA in -20 freezer but after several months the volume of the cDNA decreased to 0.1. What is the reason?
05 July 2021 7,698 1 View
Dear Friends I use BD compbeads for compensation of dyes spill over in BD FACSCalibur flow cytometer. I can't set forward scatter and side scatter of single color compbeads to have a gated beads...
01 February 2019 3,260 3 View
Thanks in advance.
31 December 2013 2,648 6 View
I used Treg Suppression Inspector (Anti-CD2, Anti-CD3 and Anti-CD28) for Lymphocyte Stimulation, but after 3 Days I see low proliferation? What,s the problem?
19 September 2013 1,791 1 View
08 September 2013 8,694 1 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View