Are there any theoretical evaluations to determine which volatile liquid can be used to dissolve a given protein with respect to reactions taking in it. If yes can anyone give me some links to find it.
What does the symbol MFZnFe5-C for finish plating means?
05 June 2013 6,605 4 View
Csv file consists of x, y, z (three colums). I have to load those three colums values into matrix & then i have to plot x vs y, x vs z. x y z 1 11 13 2 8 45 3 13 18 4...
11 December 2012 5,852 2 View
Is it possible to suck air naturally inside a chamber in a vehicle through a duct? the duct to send controlled pressure of air inside the chamber for varying speed naturally without using any...
10 November 2012 9,318 5 View
Idea of learning a analysis software.. Which among the four is best with respect to application, popularity, useage and user friendly? I learnt Ansys structural and thermal analysis but yet to...
09 October 2012 5,615 9 View
I came across liquid and gas conduction in heat transfer when I was reading about conduction. Conduction normally refers to transfer of heat taking place in one or more solids that are in contact....
09 October 2012 450 11 View
Can anyone brief me with example about the plane stress, plane strain conditions? I know that in plane stress condition Length and Breath will be more compared to thickness and so brittle will act...
09 October 2012 1,029 2 View
Is it possible to make a theoretical model to describe the statistics of a turbulent flow (in particular, its internal structures)?Also, under what conditions do smooth solutions to the...
09 October 2012 9,525 2 View
Can anyone tell me the impact factor of the journals specified below. I saw its Indexing, but I want to know whether they are Excellent or Very good or good or satisfactory or worst. Please kindly...
09 October 2012 1,333 10 View
Can anyone tell me the latest technology advancements and improvements in recent trends of KERS (Kinetic Energy Regenerative System) used in Automobile Braking?
09 October 2012 3,075 1 View
Which is the best link for viewing the impact factors of the journal. The true and the trusted one?
08 September 2012 6,374 2 View
Hello everyone, I recently submitted an article to Geotechnical Engineering (Proceedings of the ICE), and the current status has been listed as "EiC Pre-assessment: Ready" for the past 20 days. I...
10 August 2024 6,493 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
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05 August 2024 4,407 0 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...
31 July 2024 2,406 6 View
Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...
30 July 2024 2,867 0 View
Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...
30 July 2024 841 2 View