Any advice on my real time PCR amplification problem?

I am having problem with real time (SYBR green) amplification of gene. I have designed primers spanning exon-intron junction. Primer con, was 1picomole. I have used 5micrograms of RNA for cDNA...

02 March 2015 3,971 12 View

What are the best kits for miRNA isolation?

I am planning to isolate some miRNAs fro human brain tissues as well as from cell lines. Can anyone please suggest kits?

03 April 2013 6,364 4 View

Mirna prediction tools

Can anyone suggest a computational tool or software to predict mirna- mrna binding (3', 5' UTR and in ORF too.)?

03 April 2013 3,029 6 View

Primer designing for miRNA.

I am planning to detect expression of miRNAs in human tissues. Could anyone please let me know how to design primers/any online resources to design primers for miRNAs? Is it similar as we design...

03 April 2013 152 11 View

miRNA - mRNA interaction?

I want to check whether some specific miRNAs have putative binding sites in genes specifically participating in apoptosis process. Can anyone please suggest me some computational tools for the same?

03 April 2013 7,152 2 View

What is a good software to predict protein-protein or protein DNA interactions?

Can anyone please suggest tools or software for predicting putative protein-protein or protein-DNA interactions?

11 December 2012 9,647 6 View

How to enhance stability of c-DNA?

I would like to discuss various means to enhance the stability of synthesized C-DNA for further use as for PCR.

09 October 2012 7,465 3 View

Gene inhibition

Can anyone suggest what could be better option to inhibit specific gene.. Sirna or specific inhibitor compounds??

07 August 2012 3,784 2 View

Virtual PCR

Can anyone suggest any online bioinformatics tools to check theoretical amplification for primers I selected for a specific sequence?

07 August 2012 8,001 10 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View