I would like to check whether my protein of interest has putative DNA or protein binding sequences in it. Can anyone please let me know bioinformatics software and tools available for predicting the same?
I am having problem with real time (SYBR green) amplification of gene. I have designed primers spanning exon-intron junction. Primer con, was 1picomole. I have used 5micrograms of RNA for cDNA...
02 March 2015 4,086 12 View
I am planning to isolate some miRNAs fro human brain tissues as well as from cell lines. Can anyone please suggest kits?
03 April 2013 6,439 4 View
Can anyone suggest a computational tool or software to predict mirna- mrna binding (3', 5' UTR and in ORF too.)?
03 April 2013 3,117 6 View
I am planning to detect expression of miRNAs in human tissues. Could anyone please let me know how to design primers/any online resources to design primers for miRNAs? Is it similar as we design...
03 April 2013 262 11 View
I want to check whether some specific miRNAs have putative binding sites in genes specifically participating in apoptosis process. Can anyone please suggest me some computational tools for the same?
03 April 2013 7,219 2 View
Can anyone please suggest tools or software for predicting putative protein-protein or protein-DNA interactions?
11 December 2012 9,743 6 View
I would like to discuss various means to enhance the stability of synthesized C-DNA for further use as for PCR.
09 October 2012 7,544 3 View
Can anyone suggest what could be better option to inhibit specific gene.. Sirna or specific inhibitor compounds??
07 August 2012 3,855 2 View
Can anyone suggest any online bioinformatics tools to check theoretical amplification for primers I selected for a specific sequence?
07 August 2012 8,095 10 View
"PUBLISHING IN A SCOPUS JOURNAL" Researchers are now at a cross road. The critical need to publish in a Scopus or ISI, etc journal is ever vital. Journal Publication fees must be submitted....
10 August 2024 8,621 1 View
Hello everyone, I am currently developing a thesis proposal and would appreciate your input on its viability and how to effectively carry it out. My proposed topic is: "Does the perceived threat...
10 August 2024 8,992 0 View
Who will bear moral responsibility for the deaths of thousands of people in the event of an earthquake? Weeks and months remain before the onset of strong earthquakes that bring death to...
08 August 2024 6,134 12 View
There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...
06 August 2024 7,102 0 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
In the case of a wound l recurrence after radical breast cancer and sentinel lymph node biopsy. Are the sentinel lymph node procedure recommended? If no axillary lymph node dissection was not...
05 August 2024 8,056 1 View
Regarding a model for simulating battery charge and discharge, what do you consider to be high fidelity? What is the acceptable percentage of error (regardless of the metric)? Could you suggest...
03 August 2024 5,358 0 View
Hi RG family. My team and I are working on some SCOPUS publications and we need co-authors who are willing and capable of undertaking both qualitative and quantitative-based studies. The scope...
02 August 2024 7,843 0 View