10 Questions 16 Answers 0 Followers
Questions related from Ravindra Deshpande
I am having problem with real time (SYBR green) amplification of gene. I have designed primers spanning exon-intron junction. Primer con, was 1picomole. I have used 5micrograms of RNA for cDNA...
03 March 2015 4,058 12 View
I want to check whether some specific miRNAs have putative binding sites in genes specifically participating in apoptosis process. Can anyone please suggest me some computational tools for the same?
04 April 2013 7,192 2 View
I am planning to isolate some miRNAs fro human brain tissues as well as from cell lines. Can anyone please suggest kits?
04 April 2013 6,412 4 View
I am planning to detect expression of miRNAs in human tissues. Could anyone please let me know how to design primers/any online resources to design primers for miRNAs? Is it similar as we design...
04 April 2013 237 11 View
Can anyone suggest a computational tool or software to predict mirna- mrna binding (3', 5' UTR and in ORF too.)?
04 April 2013 3,086 6 View
Can anyone please suggest tools or software for predicting putative protein-protein or protein-DNA interactions?
12 December 2012 9,713 6 View
I would like to discuss various means to enhance the stability of synthesized C-DNA for further use as for PCR.
10 October 2012 7,517 3 View
Can anyone suggest what could be better option to inhibit specific gene.. Sirna or specific inhibitor compounds??
08 August 2012 3,828 2 View
Can anyone suggest any online bioinformatics tools to check theoretical amplification for primers I selected for a specific sequence?
08 August 2012 8,061 10 View
I would like to check whether my protein of interest has putative DNA or protein binding sequences in it. Can anyone please let me know bioinformatics software and tools available for predicting...
07 July 2012 5,690 0 View
