How to deal with negative values of autoradiography scans when plotting a calibration plot?

I am quantifying spots on an autoradiography scan, so subtracting the background pixels to get the intensity values but when nothing binds, it's the same as the background and since that's an...

18 August 2020 3,265 4 View

How to find/predict transcription factors for a putative promoter of the gene of my interest?

Dear All, Based on ORCA conserved regions, CpG islands, and histone methylation, I predict a putative promotor for my gene of interest with one of my colleague's help. I need to find/predict...

21 February 2020 3,035 3 View

Can a 150KDa antibody pass through 100KDa filter at 15,000g for 1 hour?

Can a 150KDa antibody pass through 100KDa filter at 15,000g for 1 hour via centrifugation?

30 September 2019 9,592 3 View

Can you fill a shell volume with SPH in LS DYNA?

Hello All! I'm finding it hard to fill the attached keyword shell volume with SPH elements. Can anybody tell me why? Is it because of the complex geometry? Thanks in advance

25 September 2019 6,886 1 View

Strain Rate in LS DYNA user defined materials?

Hello All! Anybody knows a better way than eps(i) / dt1 to calculate strain rates in a UMAT? I'm trying with eps / dt1 but apparently it is not giving me a proper rate-dependent result. Thanks..

16 July 2019 3,700 7 View

Good protocol for Mouse ESC electroporation?

Dear all, Would you kindly share with me a good protocol for mouse embryonic stem cells electroporation using Neon Transfection System? What parameters and plasmid concentrations that I should...

20 January 2019 9,045 9 View

I need a clarification regarding reverse coding of negatively-keyed items in a likert scale?

If a questionnaire includes positively and negatively-keyed items, 1)should all the negatively keyed items be reverse coded? for example, if there are two items, 1. I like the game 2. The game is...

13 September 2017 4,955 10 View

How to analyze MCQs and open-ended questions using SPSS?

30 July 2016 6,920 9 View

How is the galvanstatic polymerization of EDOT is done?

22 April 2016 2,222 1 View

Can anyone help with counting cells with fluorescence?

Hi! I have a question about how to determine cells with fluorescence in a given image (transfection efficiency calculation ). For example under a given set of conditions (expose time) cells in an...

27 July 2014 707 5 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View