How to deal with negative values of autoradiography scans when plotting a calibration plot?

I am quantifying spots on an autoradiography scan, so subtracting the background pixels to get the intensity values but when nothing binds, it's the same as the background and since that's an...

18 August 2020 3,265 4 View

How to find/predict transcription factors for a putative promoter of the gene of my interest?

Dear All, Based on ORCA conserved regions, CpG islands, and histone methylation, I predict a putative promotor for my gene of interest with one of my colleague's help. I need to find/predict...

21 February 2020 3,035 3 View

Can a 150KDa antibody pass through 100KDa filter at 15,000g for 1 hour?

Can a 150KDa antibody pass through 100KDa filter at 15,000g for 1 hour via centrifugation?

30 September 2019 9,592 3 View

Can you fill a shell volume with SPH in LS DYNA?

Hello All! I'm finding it hard to fill the attached keyword shell volume with SPH elements. Can anybody tell me why? Is it because of the complex geometry? Thanks in advance

25 September 2019 6,886 1 View

Strain Rate in LS DYNA user defined materials?

Hello All! Anybody knows a better way than eps(i) / dt1 to calculate strain rates in a UMAT? I'm trying with eps / dt1 but apparently it is not giving me a proper rate-dependent result. Thanks..

16 July 2019 3,700 7 View

Software other than ANSYS and MCalibration to get Mooney Rivlin Constants?

Hello All! I am using both ANSYS and MCalibration to get model constants for a 9 parameter Mooney Rivlin model using my uni-axial tension test data. These two software give me extremely different...

14 May 2019 352 6 View

Good protocol for Mouse ESC electroporation?

Dear all, Would you kindly share with me a good protocol for mouse embryonic stem cells electroporation using Neon Transfection System? What parameters and plasmid concentrations that I should...

20 January 2019 9,045 9 View

I need a clarification regarding reverse coding of negatively-keyed items in a likert scale?

If a questionnaire includes positively and negatively-keyed items, 1)should all the negatively keyed items be reverse coded? for example, if there are two items, 1. I like the game 2. The game is...

13 September 2017 4,955 10 View

How to analyze MCQs and open-ended questions using SPSS?

30 July 2016 6,920 9 View

How is the galvanstatic polymerization of EDOT is done?

22 April 2016 2,222 1 View

Heat exchanger with liquid Nitrogen as coolant?

I want to cool Natural Gas from 195 Kelvin to 138 Klevin in a heat exchanger by using liquid nitrogen as the coolant medium. When entering the heat exchanger, the liquid nitrogen will have a...

01 March 2021 6,450 5 View

How to get/count refractive index in gasses?

20 February 2021 8,202 6 View

What is role of toluene and stearic acid in ball milling for powder ? In what weight percent we should add this to powder?

I am doing ball milling operation of Aluminum and silicon carbide powder. I am pretty confused about what should be added as process control agent ? what is role of toluene as PCA and stearic...

12 February 2021 5,205 3 View

How to make Fast Green FCF from powder?

Hi everyone We need Fast Green to electroporate plasmid into mice embryos, therefore we simply need it to see some color in the liquid we are electroporating so that electroporation is more...

07 February 2021 1,671 2 View

How much quantity of water I have to add for wet milling of aluminosilicate refractory powder?

I want to mill Aluminosilicate refractory powder. I have 7-micrometre aluminosilicate refractory powder and I am going to do wet milling. I want to know how much water to be added to the wet...

07 February 2021 2,829 2 View

Which is the best software for the simulation of tribological properties of materials?

I want to simulate the tribological properties of solids, liquids, and fluids, please suggest good software.

02 February 2021 3,947 3 View

What is the best procedure for measuring the pH of aqueous colloidal systems?

There has long been a debate among my colleagues about measuring the pH of aqueous colloids. As I see there is no general truth in the matter, so I ask this question below. What is the best and...

31 January 2021 8,203 3 View

How to take XRD of colloidal Ag NPs?

I have synthesized silver nanoparticles using sodium citrate as reducing agent. I have carried out XRD characterization by drop coating colloidal solution on the glass substrate ( since yield in...

27 January 2021 1,416 3 View

What are the governing equations for "Impact Classifier Mill" in Powder Coating Industry?

In Powder Coating Industry, Is there any equation that relates physical properties of chips (granules of thermosetting polymer, i.e, extrudate that has been cooled and crushed) such as dimensions...

26 January 2021 1,077 3 View

Does anybody work with CoolLED in fluorescence microscopy?

I have trouble with exciting wavelengths higher than 460 nm while e.g. ex with 405 nm works. Filtercubes are correc, as I used them with my old xenon lamp before... I work on axiovert200M and with...

25 January 2021 5,201 2 View