How to deal with negative values of autoradiography scans when plotting a calibration plot?

I am quantifying spots on an autoradiography scan, so subtracting the background pixels to get the intensity values but when nothing binds, it's the same as the background and since that's an...

18 August 2020 3,265 4 View

How to separate cyclo-dopa-glucoside from betalamic acid ?

How to separate Glucoside from the acid mixture? In a particular example, I wanted to separate cyclo-dopa-glucoside from betalamic acid.

26 April 2020 1,005 11 View

How to find/predict transcription factors for a putative promoter of the gene of my interest?

Dear All, Based on ORCA conserved regions, CpG islands, and histone methylation, I predict a putative promotor for my gene of interest with one of my colleague's help. I need to find/predict...

21 February 2020 3,035 3 View

Can a 150KDa antibody pass through 100KDa filter at 15,000g for 1 hour?

Can a 150KDa antibody pass through 100KDa filter at 15,000g for 1 hour via centrifugation?

30 September 2019 9,592 3 View

Can you fill a shell volume with SPH in LS DYNA?

Hello All! I'm finding it hard to fill the attached keyword shell volume with SPH elements. Can anybody tell me why? Is it because of the complex geometry? Thanks in advance

25 September 2019 6,886 1 View

Strain Rate in LS DYNA user defined materials?

Hello All! Anybody knows a better way than eps(i) / dt1 to calculate strain rates in a UMAT? I'm trying with eps / dt1 but apparently it is not giving me a proper rate-dependent result. Thanks..

16 July 2019 3,700 7 View

Software other than ANSYS and MCalibration to get Mooney Rivlin Constants?

Hello All! I am using both ANSYS and MCalibration to get model constants for a 9 parameter Mooney Rivlin model using my uni-axial tension test data. These two software give me extremely different...

14 May 2019 352 6 View

Good protocol for Mouse ESC electroporation?

Dear all, Would you kindly share with me a good protocol for mouse embryonic stem cells electroporation using Neon Transfection System? What parameters and plasmid concentrations that I should...

20 January 2019 9,045 9 View

Can I replace a class of a classified image with another set of classes?

I have classified an image to eight classes including a shaded area class (1st classification image). Then I isolated the shaded area of the original image and classified it further into the same...

27 February 2018 7,187 4 View

I need a clarification regarding reverse coding of negatively-keyed items in a likert scale?

If a questionnaire includes positively and negatively-keyed items, 1)should all the negatively keyed items be reverse coded? for example, if there are two items, 1. I like the game 2. The game is...

13 September 2017 4,955 10 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View