So I have been getting back no mutations after amplification even though the DNA template used has mutations, what could be the reason?

13 September 2023 0 10K Report

For DNA amplification, I grow my cells in an Ampicillin+Tetracyclin culture plate. The cells used are XLBlue cells and during inoculation in LB, I add about 20uL of Amp and 25uL of Tetracyclin in a 10mL LB. For purification, Promega kit is used.

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