Kritika Ramchiary

3 Questions 6 Answers 0 Followers

Questions related from Kritika Ramchiary

So I have been getting back no mutations after amplification even though the DNA template used has mutations, what could be the reason?

For DNA amplification, I grow my cells in an Ampicillin+Tetracyclin culture plate. The cells used are XLBlue cells and during inoculation in LB, I add about 20uL of Amp and 25uL of Tetracyclin in...

13 September 2023 9,528 0 View

What is the procedure of RBC counting using flow cytommetry from a whole blood sample?

When an anti-coagulated blood sample is taken, how can we calculate the number of RBC, WBC, and platelets using the flow cytometry technique? Do we need to separate the components in the blood...

05 July 2021 224 5 View

When Ferro/Ferri redox couple is being used, why does the amount of ferricyanide taken is always more than ferrocyanide and why oxidation pot applied?

I have been using electrochemical techniques recently for a project and I have observed that the concentration of ferricyanide is always taken more than ferrocyanide. Also, oxidation potential is...

24 May 2021 7,757 9 View