Hello Everyone,
I have just started to work with animal cell culture studies. I am working on certain novel tyrosinase inhibitors as skin whitening agents. Almost all the literature survey I have done is using B16F10 cells for inhibition assays. I have SK-MEL 31 cell lines which is also melanoma cell line but from human source. I didnt come across with papers using SK-MEL lines for tyrosinase or melanogenesis inhibition. I wonder if I can use SK-MEL 31 for the same experiments.
It seems SK-MEL-31 are non-pigmented, which would mean that they are not a good model for such type of studies.
See:
https://www.jstor.org/stable/29947?seq=1#page_scan_tab_contents
I am adding the info:
CC Characteristics: Non-pigmented (PubMed=3473501).
to the Cellosaurus entry for SK-MEL-31:
https://web.expasy.org/cellosaurus/CVCL_0600
for the next release.
Best
Amos
Hello Ayşe
Actually, you can use another cell lines to detect anti-melanogenic activity including sk-mel lines such as sk-mel-3 or sk-mel-24.
I also recommend, direct enzymatic activity measurement of tyrosinase inhibition from whitening reagent.
Best Wishes
- Badges
- Science topic
- Similar topics
- Biological Science
- Molecular Cell Biology
- Culture Cells
- Cell Line