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More Yolanda Calle's questions See All

Where can I locate Latin dance DATASETS? What benefits does Latin dance provide for older adults aged 55+?

I am required to evaluate datasetsfrom other sources such as online databases and websites. However this is rather challenging to find. The evaluation of the datasets aims to inform the question...

25 June 2024 9,375 6 View

Why I have less plasmid after the digestion with SmaI?

I'm performing a digestion with SmaI but when I concentrate the digested DNA with a Zymo Kit I obtain half of the inicial DNA. I'm working at 30°C like the insert says.

30 April 2024 8,673 0 View

What is the shelf-life of Tulbaghia violacea plant extract when stored at 4°C?

I have been trying to check on literature regarding the shelf-life of a plant extract (Tulbaghia violacea) which may be used for the purposes of green synthesis, however, I have not found any...

10 April 2024 5,102 5 View

What's the pH recommended for the CaCl2 solution for bacterial transformation?

I can't find at what pH I have to adjust the CaCl2 solution. In my notes it's necessary 8.0 but i'm not sure

28 March 2024 4,651 1 View

How to improve the purity of neutrophils isolated from bone marrow using Histopaque method?

Dear all, Hello, I am recently working on isolating neutrophils from murine bone marrow by using Histopaque 1099 and histopaque 1077, but the purity of the cells are always unsatisfactory. Here I...

01 February 2024 9,203 3 View

Existen indicadores de bienestar animal en perros de trabajo?

Lo anterior relacionado para aplicar estrategias de bienestar a los perros policía, carcelarios y vigilancia y seguridad privada basados en los cinco dominios del bienestar animal: nutrición,...

13 October 2023 8,448 0 View

How to calculate the gold nanoparticle with a concentration of 6.0E+12 particles/mL to nM?

The gold nanoparticle was purchased from the company and the concentration was given as 6.0E+12 particles/mL. I was wondering if I can simply use Avagadro's number to convert it to nanomolar. The...

28 May 2023 5,738 3 View

¿Wind energy thesis?

Greetings to everyone! I had a small question. I'm currently trying to do my bachelor's thesis with a title called "Estimation of the wind potential through the computational intelligence tool for...

24 April 2023 1,982 1 View

Is there a way to calculate explained variance of node attributes in a valued ERGM?

I am fitting a valued Exponential Random Grpah Model (ERGM) of a bipartite network. I'm using R with packages ergm (from statnet), sand and network. I am using 'sum' as the valued term, the...

14 June 2022 8,790 0 View

Alternative for Transfix solution to keep Cerebrospinal Fluid?

Hello all, I was looking for an alternative solution or in-house preparation for maintaining viability of Cerebrospinal Fluid. Please if you help me.

31 January 2022 4,762 4 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

May members post flyers about opportunities to present at a conference? If so, where to post?

May members post flyers about opportunities to present at a conferehttps://veraeducation.com/nce? If so, where to post for the Virginia Educational Research Association? https://veraeducation.com/

08 August 2024 4,585 1 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Hello all, Looking for international reviewer to review Ph.D thesis in wireless sensor network.Can anybody help?

My name is Apurva Saoji. I am a Ph.D scholar in Computer engineering in India. I am looking for international expert in reviewing my PhD thesis, "Competitive Optimization Techniques to Minimize...

07 August 2024 4,600 2 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Hello Everyone ! I'm looking for a good journal to publish my manuscript with low publication cost?

I am Looking for a Science Journal with good impact factor and low publication cost to publish a review paper. Your suggestions would be appreciated.

06 August 2024 6,796 3 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Research Methodology - Impact of Corporate Reputation on Stakeholders Behaviors?

Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks

06 August 2024 949 0 View

Simona Marie Asinovski

Dear Dr. Calle,

Hello from Science Exchange! We've got a few UK labs on the platform, but you can also access US labs from the UK with total ease.

Plus, we're planning to expand into the UK- feel free to recommend some labs for us to list!

Best,

Simona

Yolanda Calle

Thank you Simona, I will have a proper look in the site.

Best wishes,

Yolanda