Here is the recipe for a standard artificial CSF that I use in brain slices. However, note that this solution requires carbogen bubbling to maintain pH at physiological level. In addition, you will need to add calcium and magnesium from 1M stocks after bubbling the the solution for 15-20 minutes, to prevent precipitation. If it is not suitable let me know and I will send you a Hepes -based recipe, which does not require carbogen bubbling. However, neurons usually are not very happy in Hepes-buffered solutions, therefore I primarily recommend this solution below.
Just in case Refik Khanjan did not precisely tell what you wanted to know: In which respects has this CSF to be kept "viable"? Is it for culturing a specific breed of cells? For studying CSF dynamics in specific, e.g., traumatological, respects? Or are you interested in something else?
Sorry, Orlando, for not realizing why you want to keep "leukocytes" viable. A resident in neurology, I did CSF leukocyte counts directly after LP. For identifying malignancies, recruiting taxi and train drivers speeded up cooled CSF Lab transfers.
In Germany, a PRAEMED-BIO Alliance seeks to improve cancer diagnostics, including flow cytometry.
There, Dr. Uwe Schedler, Geschäftsführer PolyAn GmbH, mail(at)poly-an.de, is possibly ready to give you some piece of advice.