Can zinc based fixatives be used after enzymatic dissociation of neural tissue?

I am working on a FACS experiment with mouse neural tissue. I want to preserve all cell types as much as possible and the zinc fixation papers I have read use mechanical dissociation which can...

21 January 2021 4,430 1 View

Is there a technique to store fixed cells after flow for later RNA analysis?

I am separating neuron populations from mouse brains using FACS. I am trying to find a fixation technique for intracellular antigen staining and FACS that will allow long term storage afterwards...

21 January 2021 9,722 3 View

What methodology/analysis should I use for this case?

I'm currently conducting a pre/post-test quasi-experimental research project involving a drug free program in a high school. I want to know if program-enrolled student's opinions differ from the...

28 April 2020 3,714 7 View

How to extract viral DNA from occlusion bodies?

I am trying to extract viral DNA from an NPV sample of occlusion bodies. I have used two different kits (Invitrogen Plasmind Mini Kit and Qiagen DNeasy Blood&Tissue Kit) and have not gotten...

20 June 2019 1,809 1 View

Can gene silencing/repression due to competing alleles cause PCR genotyping to fail?

I've been trying to genotype het mice with little success. I initially started with a protocol (provided and tested by the mouse strain creator) using nested primers (FWD, mutant REV, wildtype...

25 March 2019 9,965 5 View

SDS-page gel won't run?

I am new to sds gels and western blotting. My samples, ladder and just blank loading buffer will not run at all, they just sit in the well and do not move in the slightest. I got my gel and...

08 March 2016 1,463 6 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

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03 March 2021 5,360 2 View

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02 March 2021 3,060 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View