I am working on a FACS experiment with mouse neural tissue. I want to preserve all cell types as much as possible and the zinc fixation papers I have read use mechanical dissociation which can reduce viability of some cell types, mainly astrocytes. Is it possible to employ enzymatic dissociation such as papain or accutase then fix the cells with ZBF before FACS? I want to use ZBF due to its stability before analysis.
Since NOBODY answered up to now, I'd like just to add that I'm really NOT familiar with the technique you are using or going to use... Especially I'm no expert in FACS-specimen preparation....
It might be that you have or have not yet searched for specific literature, but for the sake of other colleagues dealing with similar problems (FACS, ZBF fixation etc., etc.) I would like the Google Search phrase I used to arrive at some Lit. References dealing with the 'problem':
Google Search Phrase: | FACS and ZBF OR Zinc based fixative |, and -fortunately - there were some results (and some of them might contain the answer(s) to your question already).
The first result for the convenience of the interested Reader I'd like to add as an attachment:
JENSEN et al (2010): 'Zinc Fixation Preserves Flow Cytometry Scatter and
Fluorescence Parameters and Allows Simultaneous Analysis of DNA Content and Synthesis, and Intracellular and Surface Epitopes', publ. in
Cytometry Part A - 77A: 798 - 804, 2010; DOI: 10.1002/cyto.a.20914
© 2010 International Society for Advancement of Cytometry.
NB: If this my post was not helpful at all, 'flame' separately too using my RG-messaging address.
Good luck!
STAY SAFE-STAY-WELL! - Keep your spatial DISTANCE - wash your hands quite frequently and wear your FFP2-mask: It could save a life - perhaps YOUR OWN.
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