I need a lucid protocol for the agroinoculation of geminivirus of bipartite nature which includes preparing dimeric constructs of DNA A and DNA B, triparental mating etc.
If your institute has a electroporator, it will be much faster to use electroporation to transform vectors into Agrobacteria then using triparental mating. it takes several days by using triparental mating. Electroporation takes seconds.