Silence epigenetic modifications

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How to solve the problem of unconscious teeth grinding during sleep at night?

One of my friends, every night after falling asleep, his mouth would make a creaking sound after chewing something. This teeth grinding condition would continue until he woke up, and he didn't...

08 August 2024 1,366 5 View

Is Galaxy.org good to use for research for analyzing data and for publication?

Hello all, I wanted to know, can I use galaxy (USA, Europe or Australia) platform for analyzing the shotgun data, and can it be used for publication purpose as well? Thanks :)

06 August 2024 6,610 4 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to send my account link and how to make my account public?

Nothing

01 August 2024 755 1 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Should Coefficient of Variation (CV) in a biological data necessarily be below 30%?

In a research, lesion diameter due to a disease has a CV below 10%. However, in an another parameter, disease inhibition percentage (compared to control), a value derived from lesion diameter...

27 July 2024 201 0 View

Non-parametric version of the wo-way repeated measures ANOVA?

Hi, can someone please help me with their expertise. To analyse a dataset with 2 IV´s (2 levels each) and 1 DV (time - measured at 4 points, i. e. 4 DV?) I found the two-way repeated measures...

24 July 2024 3,284 6 View

Is possible to edit a gene present in a plasmid by Crispr Cas9?

Hello everyone! Someone working with Crispr Cas9. I have a question. Is it possible to edit a gene that is present on a plasmid (in this case with a low copy number)? In this case, is it possible...

22 July 2024 7,073 2 View

How to determine tannin content in fodder pellets?

22 July 2024 8,163 3 View

To what extent are artificial intelligence techniques used to enhance science learning in schools in the Arab Gulf countries???

I want researchers who specialize in studying artificial intelligence in the field of science education and those related to it to give me information about the application of the...

18 July 2024 8,320 0 View

Which form/type of epigenetic modification is most closely associated with transgenerational effects?

Cytosine methylation, histone acetylation, etc. represent distinct epigenetic mechanisms of gene regulation. Which example is most likely to account for transgenerational effects, such as...

31 May 2024 4,613 3 View

I request an epigenetics method for DNA Methylation?

we need to study the epigenetics changes due to some genes DNA methylation

22 May 2024 5,650 2 View

What role do epigenetic modifications play in male infertility?

Can epigenetic modifications affect various aspects of sperm development, function, and fertilization?

27 April 2024 6,902 2 View

How to do CUT and TAG normalisation for EZH2 inhibited cells in H3K27me3 pulldown samples?

Dear all, I have performed CUT&TAG and tried to pulldown H3K27me3 containing nucleosomes in the cells treated with EZH2 inhibitor. I spiked my human cells with 20% of Drosophila cells. As...

21 April 2024 3,197 0 View

Can anyone advise me on epigenomic analysis?

I'm new to epigenomic analysis and have confirmed the high chromatin accessibility of a gene in tissue cells via ATAC-seq. However, I'm unsure about the specific histone modification or DNA...

13 April 2024 7,086 0 View

What can be the alternate buffer for TAPS (PH 8.5) in Post tagmentation Wash step in Cut & Tag experiment ?

I am performing Cut & Tag experiment. I was wondering if there is any alternative/replacement for TAPS pH 8.5 in single tube Tagmentation and pA-Tn5 release ( DNA purification) step of Cut and...

02 April 2024 4,214 0 View

Can a original research study analyzing real patient data be published if there have been previous papers on the genetic basis of the problem?

I am trying to find a correlation between fetal macrosomia and hypertension during pregnancy from CDC data. But there have been a lot of studies on the epigenetics of the underlying relationship...

29 February 2024 7,013 1 View

Do you think that importing meat and milk animals from abroad has an impact on the emergence of diseases or people’s behaviors?

According to epigenetic science, does it play a role in discovering genes that contain different amino acids that cause the appearance of characteristics or behaviors in people who eat meat and...

12 February 2024 4,158 1 View

What is the normal global DNA methylation level in blood? What is its level in case of breast cancer ?

Trying to find the relationship between epigenetic and cancer more deeply.

30 December 2023 8,363 2 View

What are most needed epigenetics evaluation techniques?

Dear All. If a researcher wont to conduct an article to evaluation an axpected noval epigentic compound, what is the most essential steps of techniques that should followed to optimised his...

21 December 2023 7,007 3 View