Is there any effect of different colours of light (white, red, yellow, blue etc.) on the rate of photosynthesis? If yes, why?

Light is inevitable for the process of photosynthesis reaction. Naturally sunlight plays the vital role for this process. Light may be of different colours like white, red, yellow, blue etc....

02 March 2021 7,890 2 View

What thickness need for ITO to use as conductive layer?

I want to do RF sputtering of ITO on glass substrate using magnetron sputtering. what thickness do i need to make it good conductive layer to use it for PSC. Moreover, whats about thickness of...

01 March 2021 395 3 View

What kind of food stuff increase the potentiality of human brain?

There are different kinds of food stuffs which we intake daily according to our requirements. Carbohydrate, protein, fat, mineral, vitamin, water are mainly working for the growth and development...

01 March 2021 831 2 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

Why does intensity drops as concentration increases?

Is there any reason for decrease in fluorescence emission intensity of HSA,BSA when increase the concentration ?

28 February 2021 3,653 4 View

Amorphous silica XRD peak at around 10 degree?

21 February 2021 3,417 5 View

RDF calculation of water inside the Carbon nanotubes?

I am trying to calculate the rdf of water molecules present inside the Carbon nanotube. I tried to do the indexing first using gromacs tool gmx selection. But when it comes to calculating rdf, I...

17 February 2021 1,066 3 View

What is the importance of create interfaces in 3D plaxis analysis?

While you analyse the stone columns in 3D plaxis, the interface should be used. What is the importance of that.

26 January 2021 2,440 4 View

Lighting for open field test behavior assay?

I am currently setting up our lab's behavioral space to run the open field test in rats, and I am trying to figure out how to set up lighting to evenly illuminate the open field chamber (a 90 cm x...

26 January 2021 1,399 3 View

The reason of gene silencing?

We integrated a gene in a cell line genome, when we culture them, the gene turn to silencing. We collected non-silencing cells and culture them, some of them turn to silencing again. We already...

02 December 2020 3,529 2 View

Buffy Coat preparation? What freezing media is best for ensuring optimal integrity?

Hello all. I am trying to isolate buffy coat from whole blood (sodium citrate), and planning on cyropreserving it for later analysis (~3-5 years). As to what analysis would include, we are leaving...

17 November 2020 1,899 3 View

Can any one help me to perform EWAS ?

We have conducted an experiment on epigenetic allele identification and quantification for some quantitative trait. we are trying to map epigenes using epigenome association mapping (EWAS)....

10 August 2020 4,170 3 View

How does the awareness that plants are intelligent can help plant scientists?

How does the awareness that plants are intelligent can help plant scientists? May it be a new school of thought in realm of plant studies?

29 May 2020 2,586 3 View

How to start a bioinformatic analysis of bisulfite sequencing data?

I'm just learning how to perform an analysis of methylation patterns using bisulfite sequencing data, but I have some questions: 1. What database I can use for obtain bisulfite sequencing...

10 May 2020 1,116 1 View

How to start working with epigenetic data in cancer bioinformatics?

I want to study the relationship between different epigenetic factors and the different types of cancer using existing records in epigenetic and / or oncological databases, but, as a...

28 March 2020 4,376 8 View

Can drugs change the DNA in benign disease(not in tumor)?

As we all know mutations drive cell carcinogenesis. However, does DNA change(not epigenetic modification) play a role in the occurrence of benign diseases? Such as drug complications.

17 February 2020 6,513 2 View

What are the inputs for an EWAS meta-analysis in METAL?

Hello everybody, I am new with the meta-analysis in Genome Wide Data so I have this doubt. I have read METAL documentation, which is by far the most used meta-analysis software in both EWAS and...

12 February 2020 2,922 3 View

What are the inputs for an EWAS meta-analysis in METAL?

Hello everybody, I am new with the meta-analysis in Genome Wide Data so I have this doubt. I have read METAL documentation, which is by far the most used meta-analysis software in both EWAS and...

01 February 2020 1,977 2 View

Looking for a type of non-dividing primary cells to culture?

Dear community, Happy new year! I have a very rudimental question to bother you with. I'm interested in the principles of epigenetics. All the cells I'm familiar with are in cell cycle, such as...

31 December 2019 3,318 2 View