Signal peptides in secretory proteins?

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How does public perception of vote buying affect voter behavior and participation in Ghana?

This question explores the impact of public perception of vote buying on voter behavior and electoral participation in Ghana. It seeks to understand how voters' views on the practice of exchanging...

19 July 2024 8,004 0 View

How to change reference list (muilitple pages) from APA to Harvard?

Hello, I am looking for a tool that will do a bulk conversion of references from APA to Harvard. There are over 20 pages of references, so I would like to avoid doing them one at a time. Thank...

07 July 2024 2,003 2 View

Could anyone recommend antibodies to TET2, TET3 and DNMT3B for bovine?

Can anyone recommend polyclonal or monoclonal antibodies for Tet methylcytosine dioxygenase 2 (TET2) Tet methylcytosine dioxygenase 3 (TET3) DNA methyltransferase 3 beta (DNMT3B) Ideally, they...

27 June 2024 3,557 1 View

What is the materials composition of automotibles?

What share is made of steel? Plastics and glass? Aluminum? Iron?

15 June 2024 7,246 2 View

How do I change my email address?

Today is my last day working at Missouri Department of Conservation. I have a new email address, and I don't know how to change it in my profile, so that I will continue to receive ResearchGate...

13 June 2024 8,970 2 View

Does anyone know where one can buy 8161 glass capillaries for making patch pipettes?

Warner used to sell 8161 glass, but Harvard Biosciences bought Warner and doesn’t appear to stock it anymore

04 June 2024 5,688 2 View

What are the most appropriate variables for measuring profitability of Robusta Coffee production by smallholder farmers using panel data?

Dear researchers and academicians. I am kindly requesting for your contributions to the above question on proxies for profitability of smallholder coffee production. Thanks so much and kind regards.

04 June 2024 722 0 View

How can I get a list of my publications in Research Gate and the number of Reads and other stats?

I would like to easily track which of my articles are getting reads and which are not without clicking on articles one at a time.

22 May 2024 9,047 3 View

What is the method for assessing eclipsing and ambiguity issues WQI models?

Several researchers have addressed the WQI model's uncertainty that arises due to the eclipsing and ambiguity effects on the model, and they are the major limitations of the WQI tool. Which method...

10 May 2024 1,266 1 View

T test; paired, student or independent?

Hello all: Any help appreciated. I am running an intervention with students in a esl environment. I want to compare knowledge and awareness of the topics pre and post intervention. The issue is...

14 April 2024 2,237 2 View

How to increase simulation box size?

We intend to study the interaction between peptides and polymer (like PP, PE and PS) through MD simulations using Martini force fields ( Martini 2 for PP and Martini 3 for PE, PS). We have...

08 August 2024 4,842 0 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Unusual intensity drop in some sections of chromatograms in DDA?

Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...

07 August 2024 3,218 4 View

How to analyze multiple phosphorilation sites?

Hello, I am currently analyzing some phosphoproteomics data, but I have peptides with multiple phosphorylation sites or phosphorylations together with carbamidomethylation or oxidation. How can I...

04 August 2024 8,432 3 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

Recovery rate after peptide desalting using Sep-pack C18 1 cc Vac Cartridge?

Recently I'm trying to use Sep-pack C18 1 cc Vac Cartridge (Waters: SKU: WAT054955) to desalt digested peptides. However, the recovery rate is usually 40-60%. What is the normal recovery rate?

31 July 2024 6,544 11 View

Problems with ion exchange chromatography?

I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...

27 July 2024 6,605 0 View

How do regenerate Amberlite XAD 4 resin for further cycles of use for removing 0.5% Sarkosyl from recombinant protein solutions?

I use 0.5% sarkosyl to solubilise recombinant proteins contained in Inclusion bodies of E. coli. sarkosyl very effeciently solubilizes proteins from Inclusion bodies and I am trying to use...

25 July 2024 5,001 2 View

How do nutrient transporters and aquaporins interact under abiotic stress in plants?

"The Correlation between Nutrition and Transport Mechanism under Abiotic Stress in Plants: A Comprehensive Review" by Muhammad Saleem, Jianhua Zhang, Muhammad Qasim, Rashid Iqbal, and Li Song,...

23 July 2024 9,109 0 View

Is it possible to conjugate an NHS ester to the N-terminus of a peptide ON RESIN?

So i have a few peptides (synthesized by SPPS and not cleaved from resin) and I want to add a maleimide label. We have NHS-PEG-Maleimide and from literature it is evident that the free amine group...

18 July 2024 4,075 3 View