All bacterial species can be produce enzyme?

Enzyme is a protein molecule and are biological catalysts , Microorganisms are the primary source of enzymes, because they are cultured in large quantities in short span of time and genetic...

02 March 2019 2,847 4 View

How can be used the Embryonated eggs for the viruses isolation and identification?

31 December 2018 7,432 0 View

Classification of bacteria depending of oxygen requirement ?

The environments in which some prokaryotes grow would kill most other organisms, the major physical factors which affect microbial growth are solutes and water activity, pH, temperature, oxygen...

31 December 2018 6,159 0 View

Problem in glucoamylase purification by gel filtration technique ?

31 December 2018 4,557 1 View

Determination of the glucose oxidase kinetic parameters (Km and Vmax) assay ?

The reaction rate of glucose oxidase was determined at different glucose concentration ( 20 , 12.5 , 10 , 5 , 3.33 , 2.5 ) % of glucose under optimum conditions of pH , temperature and incubation...

31 December 2018 6,532 1 View

Compare between the Azoles & Echinocandins ?

Azoles : Agents : ketoconazole, fluconazole, itraconazole, voriconazole, posaconazole, multiple topical formulations , The azoles are a broad class of antifungal agents , Echinocandins are a...

31 December 2018 4,813 1 View

Show the major nutritional types of procaryotes ?

All living organisms require a source of energy. Organisms that use radiant energy (light) are called phototrophs. Organisms that use (oxidize) an organic form of carbon are called heterotrophs or...

31 December 2018 5,300 0 View

Problem in the Optimization of the growth conditions for maximum production of the peroxidase by Bacillus subtilis?

This working was achived in Section of Biology,faculty of Science, Kufa university which contain peroxidase separation and classically of route parameters to work greatest defer of peroxidase...

31 December 2018 9,207 0 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

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02 March 2021 3,060 3 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

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28 February 2021 9,936 2 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View